粉防己碱逆转人乳腺癌MCF-7多药耐药细胞的抗凋亡作用  

Reversal of Anti-apoptotic Action by Tetrandrine in Human Breast Carcinoma Multidrug-resistant MCF-7 Cells

作　　者：王金华[1] 叶祖光[1] 孙爱续[1] 薛宝云[1] 梁爱华[1] 李春英[1] 王岚[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国中药杂志》2002年第1期46-50,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目 ( 99770 915 )

摘　　要：目的 :研究粉防己碱与长春新碱合用能否逆转人乳腺癌MCF 7多药耐药细胞的抗凋亡作用。方法 :用荧光染料Hoechst 3334 2和碘化丙锭联染观察染色质凝集 ,流式细胞术检测G1亚峰 ,TUNEL法检测凋亡细胞 ,Fluo 3染色法测定细胞内游离钙。结果 :抗肿瘤药物长春新碱处理MCF 7敏感和耐药细胞后 2 4h ,可观察到 2种类型的染色质凝集 ;但在耐药细胞中 ,相同浓度处理下染色质凝集的细胞明显减少。无明显细胞毒性的粉防己碱(2 0 μmol·L-1)与长春新碱合用 ,荧光显示法和TUNEL法均证实敏感和耐药的凋亡细胞明显增多。粉防己碱与长春新碱合用明显升高细胞内的游离钙含量。结论 :粉防己碱能有效地逆转人乳腺癌MCFObjective: To study whether the anti-apoptotic action is reversed by tetrandrine in a combination with vincristine in human breast carcinoma MCF-7 multidrug-resistant cells. Method: Chromatin condensation was observed by co-staining of fluorescent dyes Hoechst 33342 and propidium iodide; and G1 sub-peak was detected by flow cytometry. Apoptotic cells were detected with TUNEL method. Cellular free ca2+ was determined with Fluo-3 staining method. Result: Two types of chromatin condensation were observed after the sensitive and drug-resistant MCF-7 cells were treated with an antitumor drug vincristine 5 μmol·L -1for 24 h. The number of cell with chromatin condensation was obviously reduced in the drug-resistant cells treated with the same concentration of vincristine, as compared with the sensitive MCF-7 cells. The number of the apoptotic cells was increased by a combination of non-cytotoxic tetrandrine 20 μmol·L -1 and vincristine in both the sensitive and drug-resistant cells, which was confirmed with fluorescent indication and TUNEL method. The increment of introcellular free Ca2+ level in the cells treated with tetrandrine in a combination of vincristine was detected with Fluo-3 staining method. Conclusion: The anti-apoptotic action of human breast carcinoma MCF-7 cells can be effectively reversed by tetrandrine.

关 键 词：粉防已碱 长春新碱 多药耐药性 细胞凋亡 人乳腺癌 MCF-7细胞 抗凋亡作用 

分 类 号：R273[医药卫生—中西医结合]