大鼠卵巢经诊断超声照射后c-fos表达的研究  

Research on the Expression of c-fos in Rat Ovary by Irradiated with Ultrasonic Wave

作　　者：曹敏丽[1] 王怀禄[1] 赵红斌[2] 李天真[3] 尹满军 

机构地区：[1]兰州军区兰州总医院超声诊断科,兰州市730050 [2]兰州军区兰州总医院动物实验科,兰州市730050 [3]兰州军区兰州总医院病理科,兰州市730050 [4]兰州军区21军高炮旅卫生队,兰州市730050

出　　处：《中国超声医学杂志》2002年第1期13-15,共3页Chinese Journal of Ultrasound in Medicine

摘　　要：目的 :探讨大鼠卵巢经超声照射不同次数、时间对 c- fos表达的影响。方法 :将 30只雌性大鼠随机分为等同数 6组 :正常对照组、 5 min、 10 min、 2 0 min、 30 min和 6 0 min组 ,应用 EUB- 2 6黑白超声诊断仪 ,对大鼠卵巢相应部位予以不同时间持续照射 ,7天后断头处死当即取材 ,用免疫组织化学 ABC法检测 c- fos,用原位杂交方法检测 c- fosm RNA表达情况。结果 :诊断超声持续照射大鼠卵巢组织 10 min后与对照组无差异 (P>0 .0 5 ) ,照射 2 0 min后大鼠卵巢组织 c- fos表达增加并随照射时间的延长表达明显增加 ,与对照组差异显著 (P<0 .0 5 ) ,而持续照射大鼠卵巢 2 0 min时 c- fosm RNA与对照组无明显差异呈弱表达 (P>0 .0 5 ) ,而持续照射 30 min时表达增加 (P<0 .0 5 ) ,6 0 min后表达明显增加 (P<0 .0 1)。结论 :诊断超声持续照射大鼠卵巢 2 0 min后使 c- fos表达增加 ,持续照射 30 min后使 c- fosm RNA表达增加 ,从而诱导大鼠卵巢细胞凋亡。Objective:To discuss the effect of expression of c fos in rat ovary by irradiated with ultrasonic wave in different times and durations.Methods:Thirty rats were randomly divided into six groups:control,5min,10min,20min,30min and 60min group.The irradiation of ultrasonic wave was seven days,after seven days the rats were killed and the ovarian tissue was removed for detecting samples.The immunohistochemistry and ISH technology were used to detect the expression of c fos and c fosmRNA in rat ovary.Results:The expression of c fos in 10min group was not different from the control group( P >0 05);but the expression of c fos in 20min group began to increases higher than the control( P <0 05);it was the highest in 60min group than in other groups( P <0 01);on the other hand,c fosmRNA started to express in 30min ( P <0 05);there was a significant difference in expression of c fosmRNA in 60min than others ( P <0 05).Conclusions:As the duration of irradiation was prolonged longer and longer,the apoptosis of the irradiated ovarian cells was gradually induced

关 键 词：超声波 卵巢 C-FOS C-FOSMRNA 细胞凋亡 实验研究 

分 类 号：R445.1[医药卫生—影像医学与核医学]