光谱法研究Ge-132与DNA的作用机理  

Study on the Interaction Mechanism between Ge-132 and DNA bySpectral Methods

作　　者：李文友[1] 朱守田[1] 何锡文[1] 梁 宏[2] 

机构地区：[1]南开大学化学系,天津300071 [2]广西师范大学化学与生命科学学院,桂林541004

出　　处：《化学学报》2002年第1期105-108,共4页Acta Chimica Sinica

基　　金：南开大学元素有机化学国家重点开放实验室基金;广西壮族自治区教育厅科研基金资助项目.

摘　　要：利用吸收光谱、DNA碱变性曲线、荧光光谱研究了Ge-132与DNA的相互作用.在Ge-132存在下,DNA的紫外吸收光谱产生明显的减色效应.同时,Ge-132的存在使DNA碱变性的pH值增大,变性后增色效应减小.实验结果表明,Ge-132主要是以嵌入方式DNA结合的.The interaction between Ge-132 and DNA was investigated using absorption spectra, DNA alkaline denaturation and fluorescence spectra. Based on the measurements of A(260) in different pH media, the alkaline denaturation curves of CT DNA in the absence of and in the presence of Ge-132 were obtained. The results showed that the alkaline denaturation for Ge-132-DNA complex occurred at a higher pH than for DNA alone and the hyperchromicity of Ge-132-DNA complex was smaller than that of DNA, which suggested the intercalation mechanism of Ge-132 into DNA bases. All the exprimental results indicated that the intercalative binding was the major mode for the interaction of Ge-132 with DNA.

关 键 词：GE-132 DNA 嵌入作用 光谱法 分子生物学机理 抗癌药物 药物作用机理 有机错化合物 

分 类 号：O657.3[理学—分析化学]