外周天线LHCⅡ的荧光光谱特性  

FLUORESCENCE SPECTRAL PROPERTIES OF OUTER ANTENNA LHC Ⅱ

作　　者：贺俊芳[1] 王水才[1] 张舒[1] 贺锋涛[1] 任兆玉[1] 单际修[2] 李良壁[2] 匡廷云[2] 

机构地区：[1]中国科学院西安光学精密机械研究所瞬态室 [2]中国科学院植物研究所,北京100093

出　　处：《光子学报》2002年第1期6-9,共4页Acta Photonica Sinica

基　　金：国家重点基础研究专项经费 ( G1 9980 1 0 1 0 0 )

摘　　要：外周天线 LHC 在光合作用过程中 ,担负着吸收和传递光能的作用 .我们采用扫描成象荧光光谱技术对菠菜中外周天线 LHC 的荧光光谱特性进行了研究 ,在 51 4.5nm的激光激励下获取了积分荧光谱 ,认为从类胡萝卜素分子到叶绿素分子间存在有能量传递 .采用高斯组分光谱解析的方法 ,解析出 L HC 的荧光发射有七个谱带 :656.7、664 .6、671 .5、677.2、683 .5、689.6、695.3 nm,各自所占的比例分别为 3 .0 %、1 3 .1 %、 1 3 .3 %、2 1 .1 %、1 3 .2 %、3 3 .3 %、3 .0 % ,其中 658.7nm的发射谱是由叶绿素 b分子所发射的 ,其余的发射谱分别是由吸收峰为 662、670 / 671、676、680 nm以及吸收大于 690 nm的叶绿素a分子所发射 .3 .0 %的叶绿素 b分子的荧光发射说明在能量平衡过程中绝大部分能量被叶绿素 a分子所禁锢 ,689.6nm处的荧光所占的比例最大 ,可能与 L HCOuter antenna LHC Ⅱ acts to absorb and transfer energy for photosynthesis.Authors studied fluorescence properties of LHCⅡ of spinach with scanning imaging fluorescence spectroscopy.Had been excited by 514.5nm laser,the integral fluorescence spectrum of LHC Ⅱ was detected.It was shown that energy transfer existed between carotenoid and chlorophyll.Seven bands of LHC Ⅱ fluorescence emission were resolved by Gauss combination,viz.656.7,664.6,671.5,677.2,683.5,689.6,695.3nm,and the percentages of them were 3.0%,13.1%,13.3%,21.1%,13.2%,33.3%,3.0% respectively.Thereinto the emission of 658.7nm was attributed to chlorophyll b,the other emission bands were produced by chlorophyll a molecules with the maximum absorption 662,670/671,676,680nm and over 690nm.The band 656.7nm which percentage was 3.0% shown that the most energy was astricted by chlorophyll a.The percentage of band 689.6nm was the most,which was possibly correlated with one type of oneself protective mechanism of LHC Ⅱ.

关 键 词：荧光光谱 外周天线 LHCⅡ 叶绿素 光合作用 

分 类 号：Q945.11[生物学—植物学] Q63