中研Ⅰ、Ⅱ号方对猴SIV感染模型免疫功能研究  

Experimental Study of Immune Function on Monkey of SIV Infection with Zhong Yan-Ⅰand Zhong Yan-Ⅱ

作　　者：徐淑玲[1] 关崇芬[1] 王慧[1] 翁新愚[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2001年第6期32-35,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的 :中研Ⅰ、Ⅱ号复方对恒河猴感染SIVmac、SIVmac2 5 1实验观察T、B淋巴细胞增殖 ,血清 β2 -MG含量 ,T细胞亚群变化的实验研究。方法 :以猴免疫缺陷病毒SIVmac及SIVmac2 5 1感染猴 ,检测猴T、B细胞增殖变化 ,对感染猴血清 β2 -MG含量测定及对外周血CD+4T细胞百分数检测 ,观察中研Ⅰ、Ⅱ号对感染动物免疫调节作用和对免疫功能的影响。结果 :中研Ⅰ号能提高感染动物T、B细胞增殖。中研Ⅱ号提高模型动物CD4+ T细胞百分比 ,并降低动物血清 β2 -MG含量。结论 :中研Ⅰ、Ⅱ号复方均能提高感染动物免疫功能 ,并对动物免疫细胞有保护作用。In order to research the effect of ZY Ⅰ and ZY Ⅱ on simian immunodeficiency virus (SIV mac and SIV mac251), we used SAIDS model to observe the influence of T and B cell proliferation induced by mitogens before and after treatment with ZY Ⅰ. Using monoclonal antibody to label on CD + 4 T cells in PBMC with indirect immunofluorescence, the percentage was analyzed by flow cytometry. Serum β 2 microglobin (β 2MG) was measured by ELISA in ZY Ⅱ group. Results indicate that ZY Ⅰ enhanced the proliferation of T and B cells induced by mitogen, CD + 4 T cells were increased in PBMC, and serum β 2 MG level was decreased by ZY Ⅱ.

关 键 词：猴免疫缺陷病毒 Β2-MG CD4^+T细胞 细胞增殖 中研I号 中研Ⅱ号 

分 类 号：R259.129.1[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]