三七叶皂苷对气囊滑膜炎大鼠中性粒细胞功能的影响及其机制研究  

Experimental Study on the Effects of Panax Notoginsengnoside on Neutrophil Functions in Rats With Air-vesicle Synovitis and Its Mechanisms

作　　者：李晓辉[1] 李淑慧[1] 

机构地区：[1]第三军医大学药理教研室,重庆市400038

出　　处：《中国药房》2001年第11期648-649,共2页China Pharmacy

基　　金：重庆市中医药管理局科研课题 (99032)

摘　　要：目的 :研究三七叶皂苷 (PnGL)对气囊滑膜炎大鼠中性粒细胞功能的影响及其机制。方法 :以角叉菜胶 (Car)25mg/kgsc诱导大鼠气囊滑膜炎 ,分别采用试管稀释法、体外吞噬金葡菌法、细胞色素C还原法和Fura -2AM分析法 ,进行气囊灌洗液中白细胞计数、中性粒细胞 (Neu)吞噬功能、超氧阴离子 (O2· -)释放及胞内游离钙 ([Ca2 +]i)水平的测定。结果 :Car致炎后12h ,灌洗液中白细胞数量明显增多 ,Neu吞噬率和吞噬指数明显增强、释放O2· -也明显增多 ,表明Neu处于功能活化状态。与此同时 ,Neu内[Ca2 +]i水平明显升高。PnGL60～240mg/kgip 能剂量依赖性地降低灌洗液中白细胞数量、Neu吞噬功能、O2· -释放及Neu -[Ca2 +]i 水平 ,且PnGL降低Neu -[Ca2 +]i 水平的作用与其抑制Neu吞噬率及其释放O2· -的能力呈显著正相关。地塞米松 (Dex)虽能减少白细胞游出并抑制其功能活化 ,但对Neu -[Ca2 +]i 水平无明显影响。结论 :PnGL抗炎机制与Dex不同 ,降低Neu -[Ca2 +]i 水平 ,从而抑制Neu功能是PnGL发挥抗炎作用的重要分子机制之一。AIM:To study of effects of Panax notoginsengnoside(PnGL) on neutrophil(Neu) functions in carrageenin(Car) induced subcutaneous air-vesicle synovitis in rats and its mechanisms METHODS:Rat air-vesicle synovitis model was established with sc Car(25mg/kg) Neu count,the phagocytosis of Neu,the realease of O2 ·- from Neu and the level of Neu-i were measured with test tube diluting method,staphylococcus aureus phagocytosis method,cytochrome C reduction method and Fura-2 fluorescence technique respectively RESULTS:At 12h after Car used,Neu count of exudates elevated,phagocytosis rate and index of Neu and the release of O2 ·- from Neu increased obviously,suggesting that Neu was in an activated state Mea_nwhile,the level of Neu-i elevated significantly PnGL 60～240mg/kg ip,30min before and 6h after Car sc,reduced Neu count of exudates,inhibited phagocytosis functions and the increase of i level of Neu in a dose-dependent manner There was a significant correlation between the reduction of Neu-i and the inhibition of Neu phagocytosis functions and the release of O2 ·- from Neu Dexamethason had similar effects on Neu functions to PnGL,but with no effect on the level of Neu-i CONCLUSION:The reduction of Neu-i level and the subsequent inhibition of Neu functions is one of the important anti-inflammatory mechanisms of PnGL,which is different from that of Dex

关 键 词：三七叶皂苷 中性粒细胞 吞噬功能 超氧阴离子 游离钙 

分 类 号：R285.5[医药卫生—中药学]