心肌缺血过程中磷酸肌醇4-激酶变化的研究  

Study on changes in phosphatidylinositol 4-kinase activity during prolonged myocardial ischemia

作　　者：丁秀云[1] 乐加昌[2] 王士雯[1] 高雪[1] 李先锋[2] 

机构地区：[1]中国人民解放军总医院老年心血管病研究所,北京100853 [2]中国科学院生物物理研究所国家生物大分子重点实验室,北京100101

出　　处：《中华老年心脑血管病杂志》2001年第6期406-408,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家重点基础研究发展规划 973项目 ( 2 0 0 0 0 5 70 0 4) ;国家自然科学基金 (19890 380 4 )

摘　　要：目的　观察大鼠心肌缺血后心肌细胞和红细胞膜上磷酸肌醇 4 激酶活性的变化 ,探讨其在心肌缺血过程中的调控机理。方法　建立大鼠体内心肌缺血模型 ,在蛋白水平上分别检测心肌细胞和红细胞膜上磷酸肌醇 4 激酶活性在缺血后不同时间 (缺血后 1、8、2 5及 48h)的变化 ,用免疫组织化学方法检测磷酸肌醇 4 激酶蛋白表达水平的变化。结果　 (1)心肌细胞膜上磷酸肌醇 4 激酶活性在心肌缺血后 1h达到最高 ,为对照组的 6 .1倍 ,随着时间的延长 ,其活性逐渐下降 ,48h接近于正常水平 ;免疫组织化学结果显示 :与正常组相比 ,缺血组磷酸肌醇 4 激酶蛋白表达水平明显升高。 (2 )红细胞膜上磷酸肌醇 4 激酶活性在缺血后 1h和 2 5h达到最高 ,随后酶活性开始下降 ,缺血后 48h酶活性水平低于正常水平。结论　心肌缺血早期 (1h)心肌细胞和红细胞膜上磷酸肌醇 4 激酶活性显著提高 ,提示磷酸肌醇 4 激酶参与了心肌缺血后的调节过程 ,其活性升高可能与心肌受损伤程度相关。Objective To investigate the changes of phosphatidylinositol 4-kinase activity in myocardium and red blood cells during prolonged myocardial ischemia.Methods The rat in vivo ischemic model was established and phosphatidylinositol 4-kinase activity in myocardium and red blood cells was determined by isotope and immunohistochemistry at 0,1,8,25 and 48 hours after ischemia.Results The phosphatidylinositol 4-kinase activity reached its maximum in myocardium one hour after ischemia,being 6.1-folds higher than that of control and its activity declined with the lapse of time.There is obvious difference in phosphatidylinositol 4-kinase protein level between the experimental and control groups.In red blood cells,phosphatidylinositol 4-kinase activity increased to its highest level at 1 and 25 hours after ischemia,then its activity declined to the normal level at 48 hours.Conclusion It is indicated that the phosphatidylinositol 4-kinase activity increases to its highest level at the early stage of ischemia and is an important clue to the clinical diagnosis.

关 键 词：磷脂酰肌醇4-激酶 心肌缺血 红细胞膜 免疫组织化学 动物实验 

分 类 号：R540.2[医药卫生—心血管疾病]