超抗原分子SEA在肝癌细胞的表达和HLA-Ⅰ分子递呈  

Superantigen SEA expressed by human hepatocellular carcinoma cells and presented by HLA-Ⅰ molecule

作　　者：李增山[1] 隋延仿[1] 姜永强[2] 雷祚荣[2] 尚继栋[2] 

机构地区：[1]第四军医大学病理教研室肿瘤抗原肽实验室,西安710032 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《陕西肿瘤医学》2001年第1期3-5,13,共4页ShanXi Oncology Medicine

基　　金：国家自然科学基金资助项目 !编号 39770 82 7

摘　　要：目的　将超抗原葡萄球菌肠毒素A(staphylococcalenterotoxinA ,SEA)分子转入肝癌细胞以增强其免疫原性 ,开辟肝癌免疫排斥的新途径。方法　从产SEA标准菌株中获得SEA基因全长片段 ,构建SEA逆转录真核表达载体 ,通过病毒包装和滴度测定 ,最后转导肝癌细胞 ,并进行T细胞杀伤实验 ,同时利用抗体阻断的方法研究递呈途径。结果　成功的获得了表达SEA人肝癌细胞株HHCSEA。结果显示微量表达的SEA蛋白即可引发高效的免疫活性。将细胞表面的HLA I分子利用抗体阻断后 ,杀伤活性明显降低。结论　肝癌细胞表达的SEA分子具有较高的免疫激活能力 ,且有可能主要通过HLA Ⅰ分子递呈。Objective Direct transfection of hepatocellular carcinoma (HCC) cells with genes of staphylococcal enterotoxin A (SEA) could set up a new way of immunological pathway.Methods In out study, SEA gene fragment was obtained and human HCC cells wre stably tranduced with SEA gene involving the method of retroviral mediated gene transduction subsequent to construction of the retroviral vectors. Then the cytotoxicity analysis was performed with T lymphocytes and the mechanism of presentation was analyzed by blocking the HLA Ⅰ molecules.Results Human HCC cells could express SEA gene. Although tiny quantity of expression was detected, a robust immune response was promoted. After blocking the HLA Ⅰ molecules by HLA Ⅰ mAb, the cytotoxicity of T lymphocytes dropped markedly. Conclusion SEA expressed by HCC cells could elicit strong immune reaction. The results suggested that SEA could be mainly presented by HLA Ⅰ molecules.

关 键 词：肝细胞癌 超抗原 细胞毒 葡萄球菌肠毒素A 免疫排斥反应 

分 类 号：R735.7[医药卫生—肿瘤]