口服转移因子对化疗损伤小鼠免疫功能的恢复  

Effect of Oral Transfer Factor on the Recovery of Immunological Function in Mice Injured by Chemotherapeutics

作　　者：徐燕萍[1] 胡银英[1] 胡昕[1] 

机构地区：[1]江西省医学科学研究所检测中心,江西南昌330006

出　　处：《江西医学院学报》2001年第3期16-18,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的 :研究猪脾转移因子 (TF)口服途径给药对化疗损伤小鼠免疫功能恢复的作用。方法 :用环磷酰胺 (CP) 75mg/kg连续 8d给予小鼠皮下注射 ,造成免疫功能低下模型 ,同时配以口服TF 1mg/d进行治疗 (设立CP对照组 ,TF注射对照组及正常对照组 ) ,在治疗后第 8d ,分别称各组小鼠体重及脾脏 ,计算脾重指数 ,并进行外周血中性白细胞计数和用MTT法测定鼠脾淋巴细胞转化刺激指数。结果 :TF口服组体重、脾重指数 ,中性白细胞和淋巴细胞转化刺激指数多项观察指标均明显高于CP对照组 (P <0 .0 1或P <0 .0 5 ) ,与TF注射对照组比较 ,作用效果相近 (P >0 .0 5 )。结论 :口服TF能促进化疗引起的小鼠免疫功能低下的恢复 ,为其应用于临床治疗免疫功能降低性疾病提供实验依据。Objective: To investigate the effect of oral transfer factor (TF) derived from pig spleen on the recovery of immunological function in mice injured by chemotherapeutics. Methods: Cyclophosphamide (CP) was injected subcutaneously into mice (74 mg/kg) for 8 consecutively days (group A) accompanied by TF (1 mg/d) gavage (group B) or peritoneocentesis (group C). Body weight, spleen weight index and the peripheral neutrophil count were taken respectively. The blastogenesis stimulation index of mouse spleen lymphocyte was assayed by use of MTT. Results: Body weight, spleen weight index, neutrophil count and blastogenesis stimulation index of mouse spleen lymphocyte were significantly higher in group B and C than group A at day 8 ( P &lt;0.01, P &lt;0.05), and the difference between group B and C had no significant ( P &gt;0.05). Conclusions: The preparation of oral TF could promote the recovery of immunological function in mice injured by chemotherapeutics. The results provide an experimental basis for its clinical trial.

关 键 词：转移因子 口服 机体免疫功能 动物实验 免疫调节剂 

分 类 号：R979.5[医药卫生—药品]