滴金免疫技术用于HCG测定的方法研究  

A Research of Dot Immunogold Filtration Assay for Rapid Detection of HCG in Urine

作　　者：阎冀[1] 钟宏京[1] 周跃平[1] 夏惠芬[1] 沈杰[1] 

机构地区：[1]江西医学院流行病学教研室,江西南昌330006

出　　处：《江西医学院学报》2001年第3期25-27,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的 :拓展滴金免疫技术在临床应用的领域 ,并进一步优化完善该技术。方法 :用辛酸法从小鼠腹水中提取单克隆抗α hCG抗体和抗 β hCG抗体 ,用抗α hCG抗体包被胶体金 ,将抗 β hCG抗体固定在硝酸 醋酸混合纤维素酯膜 (NAC)上 ,即形成方便 ,灵敏 ,特异性强的早早孕诊断试剂盒。结果 :用该方法制得的试剂盒对HCG的检出灵敏度达到 5 0IU/L ,经 142例临床标本测定 ,阳性 98例 ,阴性 44例 ,与市售早早孕试纸条结果完全一致 ,符合率 10 0 %。结论 :通过本次研究使该方法简单、完善 。Objective: To develop the application of dot immunogold filtration assay (DIGFA) in clinic, and to consummate this method. Methods: A research was conducted by detection of HCG in urine. Two monoclonal antibodies, anti α-HCG and anti β-HCG, were extracted from rabbit by caprylic acid precipitation.A simple and rapid sensitive and highly specific method was developed by using anti α-HCG antibody to coat colloidal gold and anti β-HCG to coat on NAC membrane. Results: The detection sensitivity was 50 IU/L.142 urine samples (98 positive and 44 negative) were tested by this kit and the results were 100% in correspondence with the early pregnancy test strip. Conclusions: The method of DIGFA is simple, reliable, practical and feasible and through this study it is further consummated and more suitable for clinical application.

关 键 词：滴金免疫技术 HCG 单克隆抗体 

分 类 号：R446.61[医药卫生—诊断学]