FMLP刺激的HL-60中p38 MAPK对NADPH氧化酶p47^(phox)成分的磷酸化作用  

Effect of p38 MAPK on Phosphorylation of NADPH Oxidase Component p47^(phox) in Differentiated HL-60 Cells Stimulated with FMLP

作　　者：崔玉东[1] 

机构地区：[1]黑龙江八一农垦大学基因工程研究中心,黑龙江省密山158308

出　　处：《中国生物化学与分子生物学报》2002年第1期1-4,共4页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：为了解p38促分裂原活化蛋白激酶 (MAPK)参与NADPH氧化酶激活的机理 ,利用p38MAPK抑制剂SB2 0 35 80 ,在甲酰甲硫氨酰 亮氨酰 苯丙氨酸 (FMLP)刺激的分化为中性粒细胞样的HL 6 0细胞中研究p38MAPK对O·2 产生和NADPH氧化酶胞浆成分p4 7phox 的磷酸化作用 .实验发现 ,p38MAPK的激活过程与NADPH氧化酶的激活过程一致 .5 0 μmol LSB2 0 35 80抑制 5 0 % O·2 产生 ,完全抑制p38MAPK激活和部分抑制p4 7phox 体外磷酸化 .结果表明 ,在FMLP刺激的HL 6 0细胞中 ,p38MAPK可以通过磷酸化p4 7phox而参与NADPH氧化酶激活 .p38 mitogen activated protein kinase (MAPK) can participate in the activation of NADPH oxidase in neutrophils stimulated with formylmethionyl\|leucyl\|phenylalanine(FMLP), a chemotactic peptide. In order to understand the mechanism of activation of NADPH oxidase by p38 MAPK, effects of p38 MAPK on O · 2 generation and phosphorylation of p47 phox , a cytosolic component of NADPH oxidase,were studied with an inhibitor of p38 MAPK, SB203580, in differentiated HL 60 cells with FMLP stimulation. It was found that the kinetics of p38 MAPK activation coincided with that of NADPH oxidase activation. 50 μmol/L SB203580 could attenuate about 50% of O · 2 generation,completely inhibit the activation of p38 MAPK and partly inhibit the phosphorylation of p47 phox in vitro . These data indicate that p38 MAPK can contribute to the activation of NADPH oxidase by phosphorylating p47 phox in differentiated HL 60 cells with FMLP stimulation.

关 键 词：NADPH氧化酶 p38促分裂原活性蛋白激酶 HL-60细胞 甲酰甲硫氨酰-亮氨酰-苯丙氨酸 磷酸化 FMLP p38MAPK 

分 类 号：Q55[生物学—生物化学] Q257