利用RAPD标记构建木耳属种间关系的研究(英文)  

MOLECULAR TAXONOMIC RELATIONSHIPS OF AURICULARIA SPECIES INFERRED FROM RAPD MARKERS

作　　者：闫培生[1] 蒋家慧[1] 王东昌[1] 罗信昌[2] 周启[2] 

机构地区：[1]莱阳农学院应用真菌研究室,山东莱阳265200 [2]华中农业大学农业部农业微生物重点实验室,武汉430070

出　　处：《菌物系统》2002年第1期47-52,共6页Mycosystema

基　　金：国家自然科学基金资助项目~~

摘　　要：摘 要: 利用RAPD标记以银耳属的2个种为外类群研究了木耳属8个种的分子系统发育关系。根据UPGMA 构建的树状图结果表明木耳属(Auricularia)和银耳属(Tremella)分别为单一的分类单元。木耳属的8个种被分成3个明显不同的组,第一组包括盾形木耳(A.peltata Lloyd)和角质木耳(A.cornea Spreng.),第二组包括所有供试的黑木耳(A.auricula Underw.),第三组包括皱木耳(A.delicata Henn.)、琥珀木耳(A.fuscosuccinea Farl.)、毛木耳(A.polytricha Sacc.)、大木耳(A.maxima Y. R. Guo)和网膜木耳(A.reticulata L. J. Li)五个形态种。种间发育关系表明角质木耳和毛木耳应是两个不同的种而非同种异名,子实体横切面中髓层的有无并不表明不同种间亲缘关系的远近。Molecular taxonomic relationships of the genus Auricularia, together with outgroup of Tremella, were studied using random amplified polymorphic DNA. Eight species of Auricularia and two species of Tremella were examined. The dendrogram derived from RAPD data by UPGMA suggested that the genera Auricularia and Tremella would be monophyletic. For Auricularia, three distinct genetic groups were recognized: the first included A. peltata and A. cornea; the second included all strains of A.auricula; the third included five morphological species of A. delicata, A. fuscosuccinea, A. polytricha, A. maxima and A. reticulata. This molecular taxonomy suggests that A.cornea and A. polytricha would be two different species and not synonymous, and the presence or absence of a medulla in cross-section of the basidiocarps would not indicate whether or not taxa are closely related.

关 键 词：胶质真菌 分子系统学 异隔担子菌 木耳目 RAPD标记 柔缘关系 

分 类 号：Q949.32[生物学—植物学]