水稻遗传转化研究进展  

The Development of Rice Genetic Transformation

作　　者：王景余[1] 林秀云[2] 李明生[2] 

机构地区：[1]东北师范大学遗传与细胞研究所,长春130024 [2]吉林省农业科学院水稻研究所,公主岭136100

出　　处：《生物技术通报》2002年第1期20-25,共6页Biotechnology Bulletin

摘　　要：水稻遗传转化的主要方法 ,包括农杆菌介导法、基因枪法、电激法、PEG法等。论述了用于水稻转化的各种外植体、影响水稻遗传转化和植株分化的各种因素、各种标记基因及相应的选择方法和外源基因在转基因水稻中的遗传规律 ,比较了常用启动子在水稻中的表达强度 ,一些已转移到优良水稻品种中的有益农艺性状基因 ,如抗病、抗虫、抗除草剂等 ,介绍了它们在水稻品种改良中所发挥的作用。The methods for rice gene transformation were summarized, including Biolistics, Agrobacterium-mediation, Electroporation PEG and some other methods. Various explant for different delivery methods, factors affecting rice transformation frequency and plant regeneration, marker genes and their corresponding selection agency, the inheritance of foreign gene in transformated rice were reviewed The promoters for driving gene expression were compared. Some genes of interest have been transferred into rice, such as disease, pest, herbicide, resistant genes by different methods.

关 键 词：水稻 转基因 遗传转化 研究进展 

分 类 号：S511.03[农业科学—作物学] S188