芋脱毒苗的组培快繁及田间试验  

IN VITRO PROPAGATION AND FIELD TEST OF TARO VIRUS-FREE PLANTLETS

作　　者：柏新富[1] 蒋小满[1] 毕可华[1] 李明福 

机构地区：[1]烟台师范学院生物科学与技术系,烟台264025 [2]农业部植检所,北京100029

出　　处：《应用与环境生物学报》2002年第1期52-55,共4页Chinese Journal of Applied and Environmental Biology

基　　金：山东省科委资助项目 (No.991 1 541 0 6)~~

摘　　要：以莱阳孤芋为材料 ,研究了其茎尖分生组织培养脱毒及快繁技术 .通过酶联免疫检测和电镜观察证实 ,经 3次茎尖剥离培养所得试管苗已脱去芋花叶病毒 .脱毒试管苗在MS +ρ(BA) 1.0mgL-1+ρ(NAA) 0 .2mgL-1的培养基中培养 ,月增殖系数为 5 .6 ;而培养基MS +ρ(NAA) 0 .0 5～ 0 .1mgL-1+ρ(BA) 0 .15mgL-1,不仅适于芋试管苗的生根 ,还有利于分化成苗 .田间试验证明 ,脱毒试管苗定植于网室后 ,植株生长旺盛 ,球茎数量多 ,平均每株 2 9.1个 .两年的小区对比试验表明 ,脱毒种芋在球茎数量和产量上分别比CK增加 37.6 %～ 6 2 .6 %和 2 0 .2 %～ 40 .0 %,商品芋数增加 44 .1%～ 6 7.6 %.表 4参The virus-eliminating technology ( Colocasia esculenta Schott) with shoot-tip culture and rapid propagation of taro were studied. Two methods, i.e., the SPA-ELISA and the electron microscope, were used for virus detection of the plantlets. The results indicated that the plantlets did not carry dasheen mosaic virus with exfoliating shoot-tip ( smaller than 0.6 mm) culture by three times . The best medium for bud propagation was MS +ρ(BA)1.0 mg L -1+ρ (NAA)0.2 mg L -1, and monthly propagation index was 5.6. The rooting and new seedling differentiation were simultaneous when the shoots were cultured on MS medium supplemented with 0.05～0.1 mg L -1NAA and 0.15 mg L -1 BA. The seedlings were growing luxuriantly after virus-free plantlets were planted in the net-room, and averagely 29.1 cormels came out from each plant. Plot test showed that the amount and yield of cormels of virus-free taro were increased by 37.6%～62.6% and 20.2%～40.0%, respectively, and the amount of commodity cormels was increased by 44.1%～67.6%. Tab 4, Ref 11

关 键 词：芋 组织培养 脱病毒 田间试验 快速繁殖 脱毒苗 

分 类 号：S632.3[农业科学—蔬菜学]