基于GFP的FRET应用  

Applications of FRET based on GFP

作　　者：周炜[1] 曾绍群[1] 张智红[1] 陈同生[1] 骆清铭[1] 

机构地区：[1]华中理工大学生命科学与技术学院生物医学光子学教育部重点实验室,武汉430074

出　　处：《生命科学》2002年第1期56-58,共3页Chinese Bulletin of Life Sciences

基　　金：国家自然科学基金项目(39870205);国家杰出青年基金(60025514);高等学校博士学科点专项科研基金(980487

摘　　要：绿色荧光蛋白（ＧＦＰ）是一种活体荧光标记，已被用来研究基因表达、分子定位、蛋白质折叠和转运；荧光共振能量转移（ＦＲＥＴ）是一种无损伤的光学检测方法，能检测到小干纳米的距离变化。将ＧＦＰ的活体定位标记功能与ＦＲＥＴ的高分辨率相结合，为活体研究生物分子的功能和命运开创了新的篇章。作者在介绍 ＧＦＰ和 ＦＲＥＴ原理的基础上；综述了基于 ＧＦＰ的 ＦＲＥＴ在蛋白酶活性、蛋白质间相互作用和构象改变研究中的应用。As a genetically encoded tag, green fluorescent protein(GFP) has served as a versatile tool in cell biology for studying gene expression, molecular locating, protein folding and trafficking. Fluorescence resonance energy transfer(FRET) is a non-invasive optical method which can detect distance change as short as one nanometer. The combination of GFP technology and FRET microscope offered high(subcellular) resolution and sensitivity in living cells, and thereby creates new horizons for studying the fate and function of biomolecules in vivo. This review introduced GFP and the theory of FRET. Applications of GFP-based FRET in the study of protein-protein interactions, protein conformational changes and proteolytic processing was also summarized.

关 键 词：绿色荧光蛋白 荧光共振能量转移 构象 蛋白质相互作用 GFP FRET 应用 

分 类 号：Q51[生物学—生物化学]