HPLC法测定开胸顺气胶囊中厚朴酚与和厚朴酚的含量  

Assay of magnolol and honokiol in KAIXIONG SHUNQI CAPSULE by HPLC

作　　者：周羽琪[1] 章建民[1] 高家鉴[1] 

机构地区：[1]浙江省中医药研究院,浙江杭州310007

出　　处：《中成药》2002年第2期93-95,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :用HPLC法测定开胸顺气胶囊中厚朴酚及和厚朴酚的含量。方法 :HPLC法 ,色谱柱 :Nova PakC18,流动相 :甲醇 水 (78∶2 2 ) ,检测波长 2 94nm。结果 :厚朴酚在 0 .0 8μg 0 .4 μg ,和厚朴酚在 0 .0 5 2 μg 0 .2 6 μg ,范围内呈良好的线性关系。平均回收率 10 0 .2 2 % ,RSD为 1.2 1%。结论Objective:To determine the content of magnolol and honokiol in KAIXIONG SHUNQI CAPSULE by HPLC.Methods:Separation was achieved on a Nova Pak C 18 column. The mobile phase was metanol water(78∶22), the wavelength of the UV detector 294nm.Results:Linear range of megnolol and honokiol were 0.08μg 0.4 μg and 0.052μg 0.26μg, respectively. The average recovery was 100.22% ( RSD =1.21%).Conclusion: The method was accurate and reliable.

关 键 词：开胸顺气胶囊 厚朴酚 和厚朴酚 高效液相色谱法 HPLC 含量 中药 

分 类 号：R286.0[医药卫生—中药学]