氯胺酮-咪唑安定对感染性休克大鼠心肌HSP70表达的影响  

Effect of ketamine-midazolam on myocardial HSP 70 expression in septic shock in rat

作　　者：王泉云[1] 肖红[1] 廖刃[1] 张兰[1] 

机构地区：[1]华西医科大学附属第一医院麻醉科,成都市610041

出　　处：《中华麻醉学杂志》2001年第7期417-419,共3页Chinese Journal of Anesthesiology

摘　　要：目的　探讨氯胺酮 (KTM) 咪唑安定 (M)在感染性休克中的心肌保护作用及可能的作用机制。方法　选择健康成年SD大鼠 112只 ,随机分入对照组 (NS组、E组 )和试验组 (EM组、EK组、EKM组 )。NS组为正常对照组 ,E组给予LPS 2 0mg/kgip ,EK组、EKM组、EM组在同E组处理前分别给予KTM 80mg/kgip ,KTM 80mg/kg +M 0 5mg/kgip ,M 0 5mg/kgip ,1h后追加半剂。在LPS处理 2h后及E组大鼠临终时取心肌用免疫组化方法测HSP70的表达 ,并观察各组大鼠存活时间。结果　E组HSP70表达强于NS组 ,EK、EKM组HSP 70表达强于E组 ,存活率也高于E组 ,且随时间延长HSP 70表达呈增强趋势 ;E组、EM组HSP 70表达及存活率无明显差异。结论　氯胺酮可增强感染性休克时大鼠心肌HSP 70的表达 ,可能是氯胺酮保护感染心肌的机制之一 ;咪唑安定Objective To evaluate the myocardial protection afforded by ketamine midazolam in its septic shock and its possible mechanism by studying the impact of ketamine midazolam on myocardial HSP 70 expression in LPS induced septic shock in rats Methods 112 healthy adults SD rats of both sexes, weighing 180 205g were randomly assigned to one of following groups:(Ⅰ)control group received normal saline ip (group NS); (Ⅱ) endotoxin group received LPS 20mg·kg -1 ip(group E); (Ⅲ) endotoxin midazolam group received midazolam 0 5mg·kg -1 20 min before LPS (group EM); (Ⅳ) endotoxin ketamine group received ketmine 80mg·kg -1 20 min before LPS (group EK); endotoxin ketamine midazolam group received ketmine 80mg·kg 1 and midazolam 0 5mg·kg -1 20 min before LPS (group EKM) One hour later half of the initial dose of ketamine and/or midazolam was given to reinforce their effect Heart was harvested 2h after LPS or when the rats died of LPS induced septic shock(pupil did not respond to light) for determination of HSP 70 expression using monoclone antibody immunocytochemistry The survival time of every rat was recorded Results The expression HSP 70 was higher in group E than that in group NS and was lower than that in group EK and EKM The survival rate of group EK and EKM was higher than that of group E and EM (P<0 01) There was no diffeence in survival rate and HSP 70 expression between group EM and E or between group EK and EKM(P>0 05) Conclusions Ketamine can enhance myocardial HSP 70 expression in septic shock This may be one of the mechanisms of its myocardial protection Joint use of ketamine midazolam dose not affect the myocardial protective effect of ketamine

关 键 词：热休克蛋白70 氯胺酮 咪达唑仑 感染性休克 

分 类 号：R614.1[医药卫生—麻醉学]