东方田鼠天然抗体相关的日本血吸虫抗原基因筛选和克隆  

Screening and Cloning of Genes Encoding Schistosoma japonicum Antigens Related to the Serum Antibodies in Mirotus Fortis

作　　者：阎玉涛[1] 刘述先[1] 宋光承[1] 徐裕信[1] 何永康[2] 

机构地区：[1]中国预防医学科学院寄生虫病研究所 [2]湖南省寄生虫病防治研究所,岳阳414000

出　　处：《中国寄生虫学与寄生虫病杂志》2001年第3期153-156,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家863高技术计划 ( No.10 2 0 70 40 197);美国NIH/TMRC基金 ( No.IP5 0 A13946 1)部分资助&&

摘　　要：目的　研究东方田鼠对日本血吸虫感染天然抵抗力的蛋白分子基因。　方法　用未感染日本血吸虫的东方田鼠血清免疫筛选日本血吸虫成虫 c DNA文库 ,将阳性重组子克隆及测序。利用软件和互联网对核酸序列进行分析 ,确定目的基因。　结果　筛选出编码东方田鼠天然抵抗力相关的 7种蛋白分子基因 :三磷酸甘油醛脱氢酶、丝氨酸蛋白酶抑制剂、热休克蛋白、 2 2 .6 k Da膜相关抗原、副肌球蛋白、细胞色素 C及组织蛋白酶 B。　结论　东方田鼠对日本血吸虫的天然抵抗力可能有许多蛋白分子参与。Objective To understand and identify the molecules related to the natural resistance to Schistosoma japonicum infection in Mirotus fortis. Methods Sera from Mirotus fortis without schistosome infection were collected. The S.japonicum adult worm cDNA library was immunologically screened with the sera. The positive recombinants were identified, cloned, sequenced and analysed with software and internet. Results Seven genes encoding antigens relevant to sera antibodies in Mirotus fortis were cloned and sequenced. These antigens included glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors(SERPIN), 70 kDa heat shock protein(HSP70), 22\^6 kDa membrane-associated antigen, paramyosin (Sj97), cytochrome C and cathepsin B. Conclusion Many protein molecules might have been involved in natural resistance to \{S.japonicum\} infection in Mirotus fortis. The above 7 kinds of molecules may be identified as new candidates of vaccine against \{S.japonicum\} infection.

关 键 词：日本血吸虫 东方田鼠 CDNA文库 筛选 克隆 血吸虫病 治疗 疫苗 

分 类 号：R532.21[医药卫生—内科学]