具抗肿瘤活性的海洋微生物菌株的初步筛选  

The screening of antitumor active material from marine microorganism

作　　者：李根[1] 陈瑞川[2] 林昱[1] 

机构地区：[1]国家海洋局第三海洋研究所海洋生物工程重点实验室,福建厦门361005 [2]厦门大学抗癌中心,福建厦门361005

出　　处：《台湾海峡》2002年第1期18-22,共5页Journal of Oceanography In Taiwan Strait

基　　金：科技部基础性工作项目<海洋药源生物种质资源和基因库的构建>;国家海洋局海洋生物工程重点实验室研究课题资助 (NoHY990 3)

摘　　要：本研究采用MTT法对海洋放线菌、细菌、霉菌及极地和大洋细菌进行细胞毒活性物质的筛选 ,结果有 1 0 %放线菌及一株极地细菌具有细胞毒活性 .此外利用DNA修复特性在E .coli 3 4 3 /591和E .coli 3 4 3 /63 6之间的差异性 ,对前面筛得的菌株进行DNA损伤的筛选 ,结果得到两株活性菌株 .采用荧光染色观察得到 3株具有诱导肿瘤细胞凋亡的菌株 ,这些具抗肿瘤活性的菌株可供进一步研究 .A screening model of MTT was used to identify antitumor active material from marine actinomycetes ,bacterials,molds and extreme bacterials. The results showed about 10 percents of actinomycetes and one polar bacterial strain have cell toxicity. Further more, according to the DNA repair test vitality different between E.coli 343/591 and E.coli 343/636, two positive strains were identified. By fluorescence dyeing, we observed that three strains had the ability to induce cell apoptosis. These strains can be further researched for antitumor drug study.

关 键 词：MTT分析法 DDRT法 细胞毒活性物质 细胞凋亡 海洋微生物 海洋药物 抗肿瘤活性 

分 类 号：R822.77[医药卫生—临床医学] Q938.8[生物学—微生物学]