GABA 对大鼠下丘脑正中隆起 LHRH 释放调节的研究  

A STUDY OF THE REGULATORY ROLE OF GABA ON THE LHRH RELEASE IN THE MEDIAN EMINENCE OF RATS

作　　者：罗履广 赵白鸽[1] 赵伟[1] 朱兴族 

机构地区：[1]上海市计划生育科学研究所,上海200032 [2]中国科学院上海药物研究所,上海200031

出　　处：《生理学报》1991年第3期205-212,共8页Acta Physiologica Sinica

基　　金：国家计划生育委员会;国家人事部;世界卫生组织人类生殖处资助

摘　　要：本研究应用大鼠下丘脑正中隆起(ME),观察 γ-氨基丁酸(GABA)和去甲肾上腺素(NA)对下丘脑促黄体生成激素释放激素(LHRH)神经元末梢分泌作用的影响。结果发现:GABA(10^(-6)mol/L)可显著促进 ME 的 LHRH 和 NA 的释放,即 LHRH 释放量由27.3±2.5pg/100ul 增加至150.4±27.9pg/100μl;NA 释放量由50.9±4.2pg/100μl 增加至105.5±19.1pg/100ul,两者与对照组相比有显著差异(P<0.01)。GABA 这些作用可被受体拮抗剂荷包牡丹碱(Bicuculline)所翻转。当荷包牡丹碱和 GABA(10^(-6)mol/L)同时存在于 ME 的培灌液中,LHRH 的分泌量下降为18.2±1.9pg/100μl,而 NA 分泌量下降为43.9±3.4pg/100μl。在内源性 NA 被利血平耗竭时,LHRH 的释放量仅增加26.5%,而 GABA 能使正常大鼠 LHRH 释放量增加451.9%。本研究提示:GABA 可促进下丘脑 ME 释放 LHRH,这一作用可能通过 NA 中介。The present study was undertaken to observe the effect of GABA and NAon LHRH neuronal terminals in the median eminence(ME).The results showedthat GABA(10^(-6)mol/L)significantly increased LHRH and NA release from ME,i.e,respectively from 27.3±2.5 pg/100 μl to 150.4±27.9 pg/100 μl and from50.9±4.2 pg/100 μl to 105.5±19.1 pg/100 μl(both P<0.01 vs control group).These effects of GABA could be blocked by bicuculline.When bicuciline andGABA(10^(-6)mol/L)were added into the ME,the secretion of LHRH decreasedto 18.2±1.9 pg/100 μl and that of NA to 43.9±3.4 pg/100 μl.The effectof GABA on LHRH release may be mediated by NA.When endogenous NAwas depleted by reserpine,GABA increased LHRH secretion only by 26.5%instead of 451.9% as in the normal animal.

关 键 词：Γ-氨基丁酸 下丘脑 去甲肾上腺素 

分 类 号：R338.27[医药卫生—人体生理学]