Na^+、K^+离子通道阻滞剂对离体大鼠黄体细胞孕酮生成的影响  

EFFECT OF K+,Na+ IONIC CHANNEL BLOCKER ON PROGESTERONE PRODUCTION BY RAT CORPUS LUTEAL CELLS

作　　者：赵玉莲[1] 王京花[1] 程治平[1] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150086

出　　处：《生理学报》1991年第5期508-511,共4页Acta Physiologica Sinica

摘　　要：本工作用二种离子通道阻断剂四乙胺(TEA)和河豚毒素(TTX)来研究 Na^+、K^+通道的改变对大鼠黄体细胞孕酮生成的影响。10^(-3)mol/L 的 TEA 或 TTX 均使孕酮分泌量显著增加,而这种促进效应可被酪氨酸(Tyr)完全阻断。Tyr 对 TEA 或 TTX 与 hCG 联合所引起的孕酮分泌也有抑制作用。上述实验说明跨黄体细胞内外的 K^+和 Na^+浓度差与孕酮分泌有关。Two ionic channel blockers,TEA and TTX,were used in the present investi-gation to test whether blocking of Na^+,K^+ ion channels would affect the produc-tion of progesterone by corpus luteum(CL)cells of rat.Both TEA(10^(-3)mol/L)and TTX(10^(-3)mol/L)increased progesterone production significantly after treat-ment.This effect of the blockers could be completely inhibited by tyrosine(Tyr).In addition,Tyr was capable of reversing the combined effect of TEA,TTX andhCG on progesterone secretion.It is suggested that the concentration gradient ofNa^+ and K^+ across the CL cell membrane in experiments mentioned above isimplicated in steroidogenesis.

关 键 词：黄体细胞 孕酮 离子通道 阻滞剂 

分 类 号：R335.5[医药卫生—人体生理学]