短暂缺血预处理减轻缺血性脑损伤的研究  

Study on alleviation of ischemic cerebral injury by transient brain ischemic preconditioning

作　　者：张法永 陈衔城[1] 吴劲松[1] 鲍伟民[1] 任惠民[2] 

机构地区：[1]上海医科大学附属华山医院神经外科 [2]上海医科大学神经病学研究所

出　　处：《中华实验外科杂志》2002年第1期11-12,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　观察缺血预处理对再次脑缺血损伤的保护作用。方法　将SD大鼠分为 3组 ,分别给予生理盐水右侧颈内动脉灌注 (SI)、双侧颈总动脉夹闭 (BCAO)和双侧颈总动脉夹闭并生理盐水右侧颈内动脉灌注 (BS)。每次持续 3min ,间隔 7min ,反复 3次。 2 4h后作经插线右大脑中动脉栓塞 (MCAO) ,观察MCAO后脑组织含水量、血脑屏障通透性 (以脑伊文思蓝含量表示 )和梗死体积。结果　BS组MCAO后 2 4h和 48h脑伊文思蓝 (EB)含量和脑含水量明显较其他两组低 (P <0 .0 1) ;MCAO 72hBS组脑梗死低于其它两组 (P <0 .0 5 )。结论　双侧颈总动脉夹闭并生理盐水右侧颈内动脉灌注对脑再次缺血有明显的保护作用。Objective To investigate the influence of ischemic preconditioning on ischemic brain injury caused by subsequent middle cerebral artery occlusion (MCAO)in SD rats.Methods Male Sprage Dawley rats were divided three group.The first group(SI): Animals were only injected with saline water into right internal carotid artery ;The second group (BCAO):Animals were only treated by the method of both carotid artery occlusion;The third group(BS):Animals were given simultaneously two methods mentioned above.All experimental animals were treated three repeated procedures,3?min every time in an interval of 7?min.After 24?h,focal cerebral ischemia was made using the method of thread insertion for middle cerebral artery occlusion(MCAO).Brain water content was measured by dry wet method.Blood brain barrier permeability was determined with brain Evans blue (EB) content.Infarction size were measured by TTC staining.Results BS pretreatment decreased water content (P<0.01) and EB content (P<0.01)significantly 24?h and 48?h after MCAO.Cerebral infarction was decreased 72?h after MCAO in BS group as compared with other two groups (P<0.05).Conclusion BS pretreatment has protective effect on ischemic cerebral injury.

关 键 词：缺血预处理 缺血耐受 脑缺血 缺血性脑损伤 

分 类 号：R651.12[医药卫生—外科学]