阻断转化生长因子-β受体信号转导降低瘢痕疙瘩细胞转化生长因子-β1自分泌的研究  

Blocking TGF-β receptor signaling decreases TGF-β1 autoproduction in keloid fibroblasts

作　　者：刘伟[1] 蔡泽浩[1] 商庆新[1] 钱云良[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整复外科,200011

出　　处：《中华实验外科杂志》2002年第1期48-49,共2页Chinese Journal of Experimental Surgery

基　　金：上海市科委资助项目 (0 0JC1 4 0 32 )

摘　　要：目的　研究瘢痕疙瘩成纤维细胞转化生长因子 β1(TGF β1)的自分泌现象和阻断TGF β受体信号转导对TGF β1自分泌的调控。 方法　组织块法体外培养瘢痕疙瘩成纤维细胞 ,加入重组人源 (rh)TGF β1(5mg/L)或含缩短型TGF βⅡ型受体的重组腺病毒 (5 0 pfu/cell) ,并用Northern印迹观察它们对TGF β1极其Ⅰ、Ⅱ型受体的基因调控。 结果　rhTGF β1能上调TGF β1(3 0 %～ 5 0 % )和Ⅰ型受体 (3 0 %～ 90 % )的基因表达 ,但不影响Ⅱ型受体的基因表达。缩短型TGF βⅡ型受体在瘢痕疙瘩细胞的过度表达减少细胞TGF β1(5 0 %～ 65 % )和Ⅰ型受体 (2 1%～ 5 5 % )的基因表达 ,但不影响Ⅱ型受体的基因表达。结论　瘢痕疙瘩细胞存在TGF β1的自分泌现象 ,而缩短型TGF βⅡ型受体的过度表达可以通过阻断TGF β的信号转导来降低TGF β1的自分泌。Objective To study TGF β1 autoproduction in keloid fibroblasts and its regulation by TGF β intracellular signaling.Methods In vitro cultured keloid fibroblasts were treated with rhTGF β1(5 mg/L) or recombinant adenovirus containing a truncated type Ⅱ TGF β receptor gene (50 pfu/cell).Their effects on regulating gene expression of TGF β1 and its receptor I and Ⅱ were observed by Northern Blot.Results rhTGF β1 up regulated the gene expression of TGF β1 (30% 50%) and receptor I (30% 90%),but not receptor Ⅱ.Overexpression of the truncated receptor Ⅱ down regulated the gene expression of TGF β1 (50% 65%) and receptor I (21% 55%),but not receptor Ⅱ.Conclusion TGF β1 autoproduction is observed in keloid fibroblasts.Overexpression of the truncated TGF β receptor Ⅱ decreases TGF β1 autoproduction via blocking TGF β receptor signaling.

关 键 词：转化生长因子-Β1 自分泌 信号转导 瘢痕疙瘩细胞 纤维细胞增生性疾病 

分 类 号：R62[医药卫生—整形外科]