胆汁成核活性蛋白中糖链的致石作用  

The effect of sugar chain of vesicular protein in cholesterol nucleation process

作　　者：项建斌[1] 蔡端[1] 张延龄[1] 张群华[1] 马保金[1] 查锡良 王丽影 

机构地区：[1]复旦大学附属华山医院外科 [2]复旦大学基础医学院生化教研室

出　　处：《中华实验外科杂志》2002年第1期54-55,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (39570 695) ;卫生部科学研究项目 (98 2 1 2 4 )

摘　　要：目的　探讨糖链在胆石形成中的作用。方法　分别将 5mg辣根过氧化酶 (HRP)标记蔓陀罗凝集素 (DSA)、麦胚凝集素 (WGA)和伴刀豆凝集素 (ConA) ,鉴定糖链结构与类型 ,并在透射电镜下观察亲和染色的模拟胆汁泡形态学变化 ;通过结晶生长试验检测胆汁泡蛋白 (0 .1g/L)促成核活性及其糖链水解、多肽降解产物的活性改变。结果　胆汁泡蛋白糖链为DSA强结合、ConA(-) ,属多天线复杂型聚糖。HRP DSA标记的泡蛋白糖链 ,直接反映泡蛋白促进胆汁泡聚集、融合及胆固醇单水结晶析出的动态过程。胆汁泡蛋白具明显促成核活性 ,其It、Ig、Ic分别为 0 .5 7、1.5 2及 1.63 (P <0 .0 5 )。糖苷酶切、肽链酶解后其成核活性均几乎丧失。结论　糖链可能参与成核效应蛋白的致石作用过程。Objective To evaluate the effect of sugar chain in gallstone formation.Methods The biliary vesicular protein were examined by dot immunobinding assay of lectin coupled to a peroxidase(HRP DSA,HRP ConA,HRP WGA)and the morphology of biliary vesicles were observed under transmission electron microscopy.The protein(0.1?g/L)and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay.Results The biliary vesicular protein with multi antennary and complicated glycan displayed apparent potency of nucleation promotion,and derived crystal growth curve indices It,Ig,Ic were presented as 0.57,1.52,1.63 respectively,but after treated by N glycanase enzyme and pronase,no promoting activity were found.Conclusion The sugar chain may be involved in the nucleation process of gallstone formation.

关 键 词：糖链 胆结石 外源凝集素 胆汁成核活性蛋白 

分 类 号：R657.42[医药卫生—外科学]