γ干扰素刺激肝细胞癌细胞表达共刺激分子和主要组织相容性复合物的研究  

The study on the expression of costimulatory molecule and major histocompatibility complex by γ-interferon stimulating hepatocellular carcinoma cell lines

作　　者：吕建峰[1] 冷希圣[1] 彭吉润[1] 张佑彬[1] 牟东成[1] 翁山耕[1] 魏玉华[1] 杜如昱[1] 

机构地区：[1]北京大学人民医院肝胆外科,100044

出　　处：《中华实验外科杂志》2002年第1期76-77,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　研究共刺激分子 (CD80和CD86)和主要组织相容性复合物 (MHC)在肝细胞癌 (HCC)细胞中的表达 ,探讨γ干扰素 (γ INF)治疗HCC中的免疫作用机理。方法　应用流式细胞仪测定CD80、CD86和MHCⅡ类分子HLA DR ,以及应用微量细胞毒方法测定MHCⅠ类分子在γ INF刺激前后 8种HCC细胞中的表达。结果　所有未受刺激的HCC细胞的CD80、CD86和HLA DR表达率均不超过 3 .5 8% ,只在 3种细胞中测到MHCⅠ类分子的表达 ;γ INF以 10 6U /L的浓度刺激 48h后 ,所有 8种HCC细胞HLA DR表达率均升高 ,其中 7种升高至 18%以上 ,5种升高至 70 %以上 ,荧光强度增强 10～ 5 0倍 ;刺激后 ,在 8种细胞中均可测到MHCⅠ类分子表达 ;刺激后 ,所有 8种HCC细胞CD86表达率均升高 ,7种升高至 12 .3 5 %～ 17.10 % ,5种HCC细胞CD80表达率升高至 5 %～ 10 %。结论　γ INF刺激可使多数被研究的HCC细胞获得一定的抗原呈递能力 ,同时可使所有被研究的HCC细胞获得被细胞毒T淋巴细胞识别和杀伤的条件。Objective To study the expression of costimulatory molecules (CD80 and CD86) and major histocompatibility complex (MHC molecules) by 8 hepatocellular carcinoma (HCC) cell lines under the stimulation of γ interferon (γ INF),to investigate the mechanism of γ INF in the treatment of HCC.Methods The expression of CD80,CD86 and MHC classⅡmolecule of HLA DR by HCC cells under the stimulation of γ INF at the concentration of 10 6?U/L for 48 h was detected by flow cytometry,and the expression of MHC classⅠmolecules by HCC cells detected by microcytotoxicity.Results The expression of CD80,CD86 and HLA DR by all 8 HCC cell lines was no more than 3.58%.The positive rate of HLA DR expression by BEL7404 and HpG2 cultured for 48 h in a medium supplemented with γ INF was increased up to nearly 20%,by BEL7405 up to 70.75%,by BEL7402,QGY7701,QGY7703 and SMMC7721 up to about 95%.The positive rate of CD86 expression by 7 HCC cell lines cultured with γ INF was increased to 12.35% to 17.10%.Without the stimulation of γ INF,MHC classⅠmolecules was positive in only 3 HCC cell lines,whereas under the stimulation of γ INF all 8 HCC cell lines expressed MHC classⅠmolecules.Conclusion γ INF significantly increased the expression of HLA DR and costimulatory molecules by most studied HCC cells and improved the ability of those HCC cells to present antigens;γ INF could promote the MHC classⅠmolecules expression by all studied HCC cells and the ability of HCC cells to be recognized by cytotoxic T lymphocytes.This might be the mechanism by which γ INF induces antitumor immunity in the treatment of HCC.

关 键 词：主要组织相容性复合物 肝细胞癌 Γ干扰素 免疫调节因子 流式细胞仪 

分 类 号：R735.7[医药卫生—肿瘤]