rhIL-10在大鼠肝移植再灌注损伤和急性排斥反应中的作用  

The effect of rhIL-10 on the reperfusion injury and acute rejection in rats after liver transplantation

作　　者：邹小明[1] 杨维良[1] 姜学海[1] 王夫景[1] 聂刚[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150086

出　　处：《中华普通外科杂志》2002年第2期96-98,共3页Chinese Journal of General Surgery

摘　　要：目的我们通过大鼠原位肝移植 ,探讨了重组人白介素 10 (rhIL 10 )对肝移植后再灌注损伤和急性排斥反应的影响。方法供体为ACI(RTIa)大鼠 ,受体为LEW (RT11)大鼠。rhIL 10的注射分别为 0、2、10、5 0 μg·kg-1·d-1。结果rhIL 10 10 μg·kg-1·d-1治疗组的平均生存时间比对照组显著延长 (P <0 0 1) ,其平均血清和组织中CINC浓度与对照组相比明显减少 (P <0 0 5 )。rhIL 10治疗组于再灌注后 12h平均末梢血中性粒细胞总数与对照组相比明显减少 (P <0 0 5 )。 10 μg·kg-1·d-1治疗组在移植后 7～ 10d的末梢血中性粒细胞总数与对照组相比差异有显著意义 (P <0 0 5 )。移植后第 1天和第 7天rhIL 10治疗组的肝损伤程度和白细胞浸润程度比对照组减轻。结论rhIL 10抑制了肝移植后再灌注损伤和急性排斥反应时CINC的生成 。Objective We investigated the effect of rhIL-10 on reperfusion injury and acute rejection in rats after liver transplantation. Methods ACI (RTI a) rats and LEW (RTI 1) rats were used as donors and recipients. The dose of rhIL-10 was 0,2,10,and 50?μg·kg -1·d -1. Results rhIL-10 at a dosage of 10?μg·kg -1·d -1 significantly prolonged recipient′s survival time (13.1±1.0?d vs. 9.4±0.7d,P<0.01). The average concentration of (cytokine- induced neutrophil chemoattractant,CINC) in the serum and tissue of treated group decreased significantly compared with the control group (P<0.05). The amount of neutrophil in the peripheral blood of treated group was less than that in control group at 12 thhour after reperfusion (P<0.05). The grade of liver injuries and leukocyte infiltration in treated group was less than that in control group at 7 thand 10 thday after transplantation. Conclusion The rhIL-10 inhibits the production of CINC at the time of acute rejection and reperfusion injury after liver transplantation, and prolongs the survival time of liver grafts.

关 键 词：白细胞介素10 肝移植 再灌注损伤 移植物排斥 

分 类 号：R657.3[医药卫生—外科学] R392.4[医药卫生—临床医学]