甲基汞致小鼠肝细胞DNA的损伤  

A study on DNA damage of mouse livers induced by methylmercury

作　　者：刘晓梅[1] 孙志伟[1] 石龙[1] 

机构地区：[1]吉林大学公共卫生学院卫生化学教研室,长春130021

出　　处：《中华预防医学杂志》2002年第1期5-7,共3页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金资助 ( 395 70 60 7)

摘　　要：目的　研究甲基汞对小鼠肝细胞DNA损伤。方法　利用单细胞凝胶电泳 (singlecellgelelectrophoresis ,SCGE)技术检测 1/ 2 0 0LD50 、1/ 2 0LD50 、1/ 2LD50 的甲基汞对肝细胞DNA损伤的情况。结果　体内给予小鼠 1/ 2 0 0LD50 、1/ 2 0LD50 、1/ 2LD50 的甲基汞 ,作用 12h ,其肝细胞的存活率降低 ,DNA损伤率均增高 ,且存在明显的剂量 效应关系 ;体外给予不同剂量的甲基汞作用 1h即可使肝细胞DNA损伤率明显增高。Objective To study the DNA damage induced by methylmercury (MeHg) in mouse hepatocytes Methods The single cell gel electrophoresis (SCGE) was used to study the DNA damage of mouse hepetocytes when treated with different doses in vivo and in vitro Results After treated with high, middle and low doses of MeHg for 12 h in vivo (i p ), the proportion of DNA damage was increased and the ratio of living cells was decreased; and both effects showed significantly dose effect relationships Similar effects were found when different MeHg doses were administered to hepatocytes in vitro for 1 h Conclusion MeHg induces DNA damage in mouse hepatocytes

关 键 词：甲基汞化合物 DNA损伤 小鼠 肝 

分 类 号：R99[医药卫生—毒理学]