流加微载体半连续培养分泌HBsAg的rCHO细胞过程研究  

Semi-continuous Microcarrier Culture of rCHO Cells Secreting HBsAg by Feeding Microcarriers

作　　者：陈志宏[1] 施源[1] 丁健椿[1] 

机构地区：[1]华东化工学院生化工程研究所

出　　处：《生物工程学报》1991年第2期159-163,共5页Chinese Journal of Biotechnology

摘　　要：本文在固定浓度微载体半连续培养rCHO细胞动力学研究的基础上,通过逐步补加新的微载体以不断提高供细胞生长的表面,提高了细胞密度和产物的表达量。建立了流加微载体半连续培养rCHO细胞收获HBsAg的工艺,确定了此种培养方式的最大微载体浓度,为建立微载体连续培养rCHO细胞生产HBsAg技术,实现细胞高密度和产物高表达奠定了基础。On the basis of culturing rCHO cells semi-continuously on constantconcentration of microcarriers,the cell yield and HBsAg expression wereincreased by feeding microcarriers step by step. The process for culturingrCHO cells semi-continuously to secret HBsAg was established. Thefoundation has been laid for culturing rCHO cells continuously to reachhigh cell yield and high HBsAg expression.

关 键 词：细胞培养 微载体 HBSAG 

分 类 号：R392.2[医药卫生—免疫学]