一株人抗人A—血型物质单克隆抗体特异性的研究  

Studies on the specificize of a Human Monoclonal Antibody Against A Blood Group Substance

作　　者：陈华堂[1] Larrick J Kabat E.A 

机构地区：[1]安徽医科大学生化教研室 [2]Cetus lmmune Corporation Palo Alto CA94303 [3]Departments of Microbiology Genetics and Development and Neurology, College of Physicians and Snrgeona Columbia University New Yolk N. Y. 10032. U. S. A.

出　　处：《生物化学杂志》1991年第1期110-116,共7页

基　　金：国家教委<优秀年轻教师基金>

摘　　要：本文对一株人抗人A-血型物质单克隆抗体,用定量免疫沉淀法以及ELISA研究其与多种单糖、双糖及寡糖的反应性,从而确定了其结合部位的结构特异性。实验发现其结合部位互补于含有双分子岩藻糖残基的A-t糖:这一研究进一步强调了含有双分子岩藻糖残基的A血型抗原决定簇的重要性。This is the first human monoclnal antibody against A blood group substance, generated by Epstein-Barr virus trans-formation of huma lymphocyte isolated from splenic tissue after activation in vitro with group A blood cells.the antibody has been characterized by quantitative precipitin assays and the fins structure of its combining site has been mapped by quantitative ELISA assays by using A-active oligosaccharides to inhibit the binding of anti-A and A blood group substance. The human monoclonal anti-A strongly precipitated various A-active blood group substances, but not B, H, Lea, and the precusor of Ii substances.The combining site was most complemntary to the difucose containing A-Hep tasaccharide:GalNAcα1→3Galβ1→3GlcNAcβ1→3Galβ1→4Glc Fuca FucaThe se studies futher emphasize the importance of difucosyl determinants.

关 键 词：单克隆抗体 结合部位 抗原决定基 

分 类 号：R392.11[医药卫生—免疫学]