小鼠肝脏金属硫蛋白Metallothioneins的分离纯化与鉴定  

Purification and Characterization of Mouse Liver Metallothioneins

作　　者：茹炳根[1] 潘爱华[1] 王正新[1] 张龙翔[1] 

机构地区：[1]北京大学生物学系,北京100871

出　　处：《生物化学杂志》1991年第3期285-290,共6页

基　　金：国家863-103-20-11;75-71-01-10;自然科学基金3860016;THE THIRD WORLD ACADEMY OF SCIENCES RE8EARCH GRANT NO.48-CHN 6的资助

摘　　要：由健康小鼠通过皮下注射一定量的氯化镉,取肝脏匀浆,离心,经SephadexG-50和DEAE-Sephadex A-50柱层析分离,即可获得MT-Ⅰ和MT-Ⅱ两种金属硫蛋白。经鉴定:两个分子各含18个巯基,结合4个Cd原子和3个Zn原子,无金属蛋白质Thionein的分子量约为6kD,半胱氨酸含量约占氨基酸总量的28％,所提取到的MT-Ⅰ和MT-Ⅱ经氨基酸组成,HPLC和PAGE分析皆证明为高度均一的,且与有关文献报道基本相符。其中MT-Ⅱ已获得晶体。Metallothioneins (MT- Ⅰ and MT- Ⅱ) are producted from Swiss mouse liver induced by subcutaneous injection with cadmium chloride and isolated by means of Sephadex G-50 and DEAE-Sephadex A-50 column chromatography. Even molecule of metallothionein contains 18 mercapto groups and combines with 4 cadmium atoms and 3 zinc atoms. The molecular weight of thionein (no metal) is about 6kD, The content of cysteic residues is 28% in the all of amino acid residues. Purified MT-Ⅰ and MT-Ⅱ are shown to be highly homogeneous by analysis of amino adid composition, HPLC and PAGE. MT-Ⅱ has been crystallized.

关 键 词：肝脏 金属硫蛋白 

分 类 号：Q593.103[生物学—生物化学]