光标生物素HBV-DNA探针及其临床应用  

Study on Photoactive Biotin Labeled HBV DNA and Its Clinical Usage

作　　者：杜绍财[1] 陈苹[1] 许向东[1] 刘金祥[1] 陶其敏[1] 王来新 邱淑燕[2] 王春光[2] 张礼和[2] 

机构地区：[1]北京医科大学肝病研究所,北京100083 [2]北京医科大学药学院

出　　处：《生物化学杂志》1991年第4期431-435,共5页

摘　　要：本文报告了应用生物素交联光敏补骨酯素(Bp,Biotin-Psoralen)标记HBV DNA探针的动力学研究和临床应用结果。表明化学合成Bp经366nm光照与DNA发生加成反应。能标记dsDNA,也能标记ssDNA。加热可使Bp-dsDNA变性,强碱则不能使Bp-dsDNA变性。临床DNA检测结果表明,应用Bp-HBV探针杂交,DNA检出率与HBeAg符合率达99.08％,与^(32)P-HBV符合率达98.8％,提示光标记Bp-HBV探针灵敏度接近同位素探针。This article reports the dynamic research and clinical usage of the HBV DNA probe labeled with biotin binding psoralen(B-p) . It turns out hat B-p can react with DNA under 365nm UV irradiation. Not only can B-p cross-linking with dsDNA, it also can form adduct with ssDNA. When heated, B-p dsDNA is denatured through the photoreversible reaction, while the strong base shows no effect to the complex. The results of clinical using show that, when the B-p is used as hybrid probe to detect DNA, the sensitivity is comparable to that of isotope probe.

关 键 词：DNA探针 生物素 补骨酯素 HBV 

分 类 号：R446.62[医药卫生—诊断学]