基质金属蛋白酶在类似人类2型糖尿病大鼠肾病中的研究  

Studies on changes of the MMP-2 and TIMP-2 in kidney of the type 2 diabetic rat

作　　者：段文澜[1] 方京冲[1] 杨秀芳[1] 史虹莉[1] 朱禧星[1] 陈文平[2] 张志刚[2] 

机构地区：[1]上海医科大学附属华山医院糖尿病研究室,200040 [2]上海医科大学病理解剖教研室

出　　处：《中国糖尿病杂志》2001年第5期290-294,共5页Chinese Journal of Diabetes

摘　　要：目的　动态研究基质金属蛋白酶 - 2 (MMP- 2 )和其体内特异的抑制物 -金属蛋白酶组织抑制剂 - 2 (TIMP- 2 )在类似人类 2型糖尿病大鼠肾组织的改变。方法　利用免疫组织化学观察 型胶原在肾小球的表达 ,酶谱法测定肾皮质MMP- 2的活性 ,蛋白印迹法检测肾皮质 TIMP- 2的含量 ,Northern Blot观察肾皮质 MMP- 2和 TIMP- 2的基因表达。结果　类似人类 2型糖尿病大鼠肾小球 型胶原成分表达增强 ;肾皮质 MMP- 2基因表达减弱 (P <0 .0 1) ;其活性进行性减少 (P <0 .0 0 0 1) ,而 TIMP- 2基因表达 (P <0 .0 1)及其含量逐渐增加 (P <0 .0 1)。结论　类似人类 2型糖尿病大鼠肾组织 MMP- 2活性进行性降低、TIMP- 2含量增加 ,两者比例失衡为肾小球 ECM成份沉积的重要原因之一 ,基质降解减弱也参与了Objective To study the changes of the MMP 2 and TIMP 2 in kidney of the rat with type 2 diabetes at different stages.Methods Immunohistochemistry was used to evaluate the content of type Ⅳ collagen in glomerulus. In renal cortex, zymography was used to investigate the gelatinolytic activity of MMP 2. Western Blot was used to evalulate the content of TIMP 2, and Northern Blot was used to observe the gene expression of MMP 2 and TIMP 2. Results In renal cortex from the type 2 diabetic rats, type Ⅳ collagen staining in glomeruli was increased at the 12th and 20th week; gene expression of MMP 2 was reduced and the gelatinolytic activity impaired; meanwhile the gene and protein expressions of TIMP 2 were progressively increased.Conclusion The impairment of the matrix degradation in renal tissue plays an important role in the development of diabetic nephropathy in type 2 diabetes.

关 键 词：2型糖尿病 肾病 细胞外基质 基质金属蛋白酶-2 金属蛋白酶组织抑制剂-2 

分 类 号：R587.1[医药卫生—内分泌]