抗纤软肝颗粒对PDGF诱导下HSC细胞周期及胞内Ca^(2+)浓度的影响  

Effects of Kangxian Ruangan Granule on Platelet Derived Growth Factor (PDGF) Induced Cell Cycle and Intracellular Calcium Concentration of Hepatic Stellate Cells

作　　者：杨玲[1] 程红球[1] 朱清静[1] 张赤志[1] 

机构地区：[1]湖北中医学院附属医院肝病中心,武汉430061

出　　处：《中西医结合肝病杂志》2002年第1期20-22,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省自然科学基金(课题编号:2000J042);湖北省教育厅(课题编号:2000A06010)

摘　　要：目的:研究抗纤软肝颗粒对血小板衍生生长因子(PDGF)诱导的肝星状细胞周期及胞内Ca^(2+)浓度的影响。方法:采用完全无血清培养,用流式细胞术分析抗纤软肝颗粒对PDGF诱导的HSC增殖的影响,应用Ca^(2+)荧光指示剂Fura-2/Am在荧光分光光度计上测定细胞内Ca^(2+)浓度。结果:与对照组相比,抗纤软肝颗粒处理组可显著地抑制PDGF诱导的HSC增殖及细胞内Ca^(2+)浓度的升高,尤以抗纤软肝颗粒5mg/ml组作用明显。结论:抗纤软肝颗粒能拮抗PDGF诱导的细胞内Ca^(2+)浓度的升高。Objective: To study the effect of Kangxinan Ruangan Granule (KXRGG) on platelet derived growth factor induced the cell cycle and intracellular calcium concentration. Methods: Flow cytometry and Calcium probe were used to analyse the proliferation and intracellular calcium concentration of HSC cultured in an absolutely serum free medium. Results s PDGF induced the proliferation of HSC and the increasing of intracellular calcium concentration were inhibited significantly in KXRGG treated group, especially in 5mg/ml KXRGG group as compared with control group. Conclusion: KXRGG inhibits the proliferation of HSC by influencing the intracellular concentration of calciam induced by PDGF.

关 键 词：血小板衍生生长因子 肝星状细胞 细胞内CA^2+ 细胞周期 抗纤软肝颗粒 中药 

分 类 号：R285[医药卫生—中药学]