大黄对创伤后脓毒症大鼠肝细胞线粒体功能的影响  

The effects of rhubarb on the function of mitochondria of hepatic cell in posttrauma sepsis

作　　者：陈德昌[1] 李红江[1] 乔林[1] 杨兴易[1] 景炳文[1] 

机构地区：[1]第二军医大学附属长征医院急救科,上海200003

出　　处：《中国中西医结合急救杂志》2002年第1期9-11,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目 ( No.395 70 6 88)

摘　　要：目的 :研究大黄对烫伤和内毒素“二次打击”模型大鼠肝细胞线粒体功能的影响。方法 :采用大鼠背部烫伤模型 ,烫伤后 12小时静脉内给予内毒素 2 0 m g/ kg进行“二次打击”。分离肝细胞线粒体 ,监测肝细胞线粒体内 ATP水平、细胞色素 C含量和能荷。结果 :烫伤能引起肝细胞内 ATP含量明显减少 ,细胞色素 C和能荷明显下降 ;内毒素打击后上述指标降低更显著 ,且此过程随时间而加重 ,各时间点与正常对照组均有显著性差异 (P均 <0 .0 0 1) ;大黄可明显提高脓毒症大鼠细胞内 ATP含量和能荷值 ,减少肝细胞内细胞色素 C的丢失 ,与单纯烫伤组和脓毒症组比较有非常显著性差异 (P均 <0 .0 0 1)。结论 :烫伤和内毒素“二次打击”能导致肝细胞内 ATP和细胞色素 C含量明显减少 ,能荷下降 ,而大黄能明显提高肝细胞内 ATP和细胞色素 C含量和能荷 ,改善肝细胞线粒体的呼吸功能。Objective:To investigate the effects of rhubarb on the function of mitochondria of hepatic cell in posttrauma sepsis.Methods:One hundred and eight SD rats with 25% Ⅲdegree burns on back hit by endotoxin at a dose of 20 mg/kg 12 hours after the burn injury.Mitochondria was isolated from the hepatic cells.The level of ATP,cytochrome C,and energy charge in hepatic cells were applied to evaluate the function of mitochondria.Results:The level of ATP,cytochrome C,and energy charge in hepatic cells was decreased after burn injury,and deteriorated further after the second hit by endotoxin on a time manner.There were significant differences compared to the controls (all P <0 001).However,rhubarb could obviously increase the level of ATP,cytochrome C and energy charge in hepatic mitochondria,the differences were significant compared to the burn and septic group (all P <0 001).Conclusions:Burn injury and the postburn sepsis,hit by endotoxin,can damage the function of mitochondria in hepatic cells.Whereas,rhubarb obviously improved the mitochondrial respiration of hepatic cells in the postburn sepsis.

关 键 词：大黄 内毒素 线粒体 烫伤 肝脏 中药 药理 

分 类 号：R285.5[医药卫生—中药学]