神经生长因子对脊髓损伤后脊髓组织一氧化氮生成的影响  

Effects of Nerve Growth Factor on Nitric Oxide (NO) Production after Spinal Cord Injury in Rats

作　　者：汤长华[1] 曹晓建[1] 王道新[1] 

机构地区：[1]南京医科大学第一附属医院骨科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2001年第5期375-377,F002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目 ( No.3980 0 176)

摘　　要：目的 :探讨神经生长因子 ( NGF)对脊髓损伤保护作用的机制 ,为临床应用提供理论依据。方法 :采用 Allen法以 2 5 g/cm3力致伤大鼠 T8脊髓 ,插蛛网膜下隙导管于术后即刻、2、4、8、12、2 4 h各注入神经生长因子 ( NGF)溶液 ,并与生理盐水组和正常对照组作对照。采用免疫组化法 ( ABC法 )和原位杂交法检测神经元型固有型一氧化氮合成酶 ( nc NOS)在脊髓中的表达。结果 :大鼠伤后脊髓前角运动神经元出现 nc NOS蛋白和 nc NOS m RNA异常表达 ,NGF组与生理盐水组相比较 ,nc NOS蛋白和nc NOS m RNA异常表达明显减少 ( P<0 .0 1或 P<0 .0 5 )。结论 :NGF能通过抑制脊髓损伤 nc NOS的异常表达 ,抑制一氧化氮( NO)过多释放所致的神经毒性作用 ,从而保护了损伤的神经组织。Objective:To explore the protective mechanisms of nerve growth factor (NGF) on spinal cord injury and provide theoretical basis for its clinical usage. Methods:The spinal cord injury (SCI) of Wistar rats was performed with Allen′s way by a 25 g/cm 3 impact on the posterior T 8 spinal cord. NGF was given to the treatment group by tube, which implanted into subarachnoid space at once and at 2?4?8?12?24 hours after spinal cord injury. The neuronal constitutive nitric oxide synthase (ncNOS) protein levels in rat spinal cord were detected by the method of immunohistochemistry. The cNOS mRNA expression was studied with in situ hybridization. Results:Expression of ncNOS and ncNOS mRNA were induced in spinal ventral horn motorneuron in injuried rats, as compared with that in control group. The levels of ncNOS protein and ncNOS mRNA in NGF group were significantly lower than that in control group ( P<0.01 and P<0.05 seperatively). Conclusion:NGF can protect spinal cord from injury in vivo. NGF may have a role to inhibit abnormal expression of nitric oxide synthase (NOS), and indirectly inhibit the neurotoxicity of NO.

关 键 词：脊髓损伤 神经生长因子 一氧化氮 免疫组织化学 原位杂交 

分 类 号：R651.2[医药卫生—外科学]