血管生成抑素的原核表达及纯化  

Prokaryotic Expression of Angiostatin

作　　者：傅赞[1] 吴月[2] 李艳丽[2] 朱自路[2] 赵翰林[1] 陈丙莺[2] 

机构地区：[1]南京医科大学第一附属医院外科,江苏南京210029 [2]南京医科大学生物与分子生物学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2001年第5期403-405,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的 :构建血管生成抑素 ( angiostatin)的原核表达载体 ,并转入大肠杆菌 BL 2 1中诱导表达并纯化。方法 :设计引物扩增 angiostatin的 c DNA,然后亚克隆入原核表达载体 p ET3 2 a,并转化入大肠杆菌 BL2 1中诱导表达。结果 :通过重组质粒酶切和测序分析等方法 ,筛选出重组阳性克隆 ,转化入大肠杆菌 BL 2 1中诱导表达 ,并纯化成功。结论 :通过构建的原核表达 ,获得了纯化的Objective:To construct the expressive vecto r of angio statin and transform into E. coli BL21(DE3), to induce the expression of recombi nant angiostatin in prokaryotic system. Methods:The cDNA of Angiosta ti n was amplified by PCR, they were subcloned into vector, and transformed into th e E. coli BL21(DE3), and induced expression of recombinant angiostatin. Re sults:The recombinant clones were picked out by the restriction endonuclea se and sequence analysis. Recombinant angiostatin was highly expressed when the st rain was induced with IPTG(1 mmol/L), and angiostatin was successfully purified. Conclusion:The recombinant angiostatin can be used in further studi es.

关 键 词：生长因子 血管生成抑素 原核表达 纯化 肿瘤 

分 类 号：Q782[生物学—分子生物学] R322.12[医药卫生—人体解剖和组织胚胎学]