液相色谱法分离纯化固相合成的胸腺素α1  

Study on the Separation and Purification Techniques of Solid-phase Synthetic Shymosin α1 by the Liquid Chromatogram

作　　者：黄鹤[1] 万里鹏[1] 吴蕾[1] 甘一如[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《化学工业与工程》2001年第6期331-335,353,共6页Chemical Industry and Engineering

基　　金：天津市自然科学基金资助项目 (99380 4 311)资助

摘　　要：本文为以Fmoc(Nα-芴甲氧羰基 )固相合成法制备的胸腺素α1粗品提出了一条实验室规模的液相色谱法纯化路线 ,共分两步 :第一步采用DEAESepharoseFastFlow填料进行离子交换层析操作 ,分离除去粗品中的大部分杂质 ;第二步用制备型反相高效液相色谱 (用C1 8色谱柱 )进行最后的纯化。终产物纯度可达 95% ,生物活性符合实验指标。In this paper,a purification route using the liquid chromatogram on a loboratory scale was established on thymosin α1 synthesized by the solid-phase synthetic method using Fmoc group fo protecting α-amino acid.Two steps were included in the purification route.First was the Ion-exchange chromatography (IEC) using DEAE Sepharose Fast Flow as the matrix.After this step,majority of impure peptides was removed.Second was the preparation RP-HPLC on C18 column.Then the final product's purity attained to 95% and the values of the biological activity all meet the requirements.Those operation conditions during the separation and purification process were also studied and optimized in this thesis.

关 键 词：胸腺素Α1 固相合成多肽 离子交换层析 反相高效液相层析 分离纯化 

分 类 号：TQ287.2[化学工程—有机化工] O629.72[理学—有机化学]