气相扩散速率对溶菌酶晶体生长的影响  

The Effect of Vapor Diffusion Rate on Lysozyme Crystal Growth

作　　者：戴国亮[1] 董向群[1] 孙祉伟[1] 胡文瑞[1] 

机构地区：[1]中国科学院力学研究所国家微重力实验室,北京100080

出　　处：《物理化学学报》2002年第1期70-73,共4页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金(19789201);攀登计划(95-预-34)资助项目~~

摘　　要：为研究气相扩散速率对溶菌酶晶体生长的影响,特设计了一种可方便调节气相扩散速率的晶体生长样品池,并用动态光散射法对不同气相扩散速率下晶体生长过程进行了研究.实验结果表明,随着气相扩散速率的增加,晶体生长过程中的成核阶段缩短.气相扩散速率通过影响溶菌酶溶液形成过饱和的速率来影响生长出的晶体的数量和大小.A sample cell was specially designed and improved for studying crystal growth of lysozyme using vapor diffusion approach by dynamic light scattering.Various rates of vapor diffusion can be easily achieved by adjusting the concentration of the precipitator.A series of experiments using 1,3 and 4 mol·L-1 NaCl solution and silica gel particles as precipitator were performed and lead us to the conclusion that the rate of vapor diffusion was of great importance for controlling crystal growth.For higher precipitator concentrations (3,4 mol·L-1 NaCl solutions and silica gel particles),the sizes of particles in the initial stage fluctuate very considerably,while it was not the case for lower concentrations (1 mol·L-1 NaCl solution).During the nucleation stage,the time period decreased with the increasing concentration of the precipitator,but the diameters of particles remained at about 4～6 nm in despite of different rates of vapor diffusion.This shows that the rate of forming supersaturation plays an important role in crystal growth process.

关 键 词：气相扩散 溶菌酶 晶体生长 动态光散射 蛋白质 扩散速率 

分 类 号：O782[理学—晶体学]