肺炎克雷伯氏菌培养工艺的研究  

Study on Culture Technological Conditions of Klebsiella Pneumoniae

作　　者：方文[1] 吴蕾[2] 张瑛[2] 甘一如[2] 葛新[3] 

机构地区：[1]天津大学化工学院,天津,300072；天津医科大学,天津,300070 [2]天津大学化工学院,天津,300072 [3]天津医科大学,天津,300070

出　　处：《化学工业与工程》2002年第1期6-11,共6页Chemical Industry and Engineering

基　　金：天津市科委资助项目 ;( 993 0 0 4 4 11)

摘　　要：本文研究了肺炎克雷伯氏菌的培养工艺 ,得到了实验范围内最佳的培养基组成和培养条件 :葡萄糖 1% (质量分数 ,下同 )、大豆胨 1 5 %、牛肉浸膏 0 2 %、酵母粉0 2 %、蛋白胨 0 5 %、氯化钠 0 5 %、K2 HPO40 35 %、KH2 PO40 15 %。 2 %接种量 ,转速16 0r min和 2 0The cultivation of Klebsiella pneumoniae in flask was studied. The medium recipe suitable to Kp-9 strain growth and expression of the capsular glycoprotein was proposed. The ingredients of the recipe include glucose 1%, soya peptone 1.5%, beef extract 0.2%, yeast extract 0.2%,tryptone 0.5%,NaCl 0.5, K 2HPO 4 0.35% and KH 2PO 4 0.15%. The suitable culture conditions are 160r/min of rotate speed, 20% of filling capacity and 2% of inoculum volume.

关 键 词：肺炎克雷伯氏菌 荚膜糖蛋白 培养 工艺条件 

分 类 号：TQ464.7[化学工程—制药化工]