棕色棉细胞质雄性不育花药的细胞学观察  

Cytological observation of cytoplasmic male-sterile anther of brown cotton.

作　　者：李悦有[1] 王学德[1] 徐亚浓[1] 

机构地区：[1]浙江大学农学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2002年第1期11-15,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家转基因植物研究与产业化开发专项 ( J0 0 -B- 0 0 2 - 10 ) ;国家高技术研究发展计划 ( 863计划 ;2 0 0 1AA2 12 0 81) ;国家自然科学基金 ( 3 0 170 0 5 8);国家教育部科技研究基金 (重大 0 114 )资助项目

摘　　要：对棕色棉细胞质雄性不育系和保持系的小孢子发生进行了细胞学观察 .结果表明 ,棕色棉不育系小孢子发生败育的时期是在造孢细胞增殖至小孢子母细胞进行减数分裂的整个过程中 .造孢细胞和小孢子母细胞退化导致雄性不育的主要细胞形态学特征是 :造孢细胞核仁增大、细胞质液泡化和细胞形状畸形 ,还有因核仁物质弥散而使整个细胞染色较深 ;小孢子母细胞核仁穿壁普遍、多核仁、染色体行为异常、细胞互相粘连成巨型团块和液泡化 。Cytological observation of microsporogenesis was performed for a cytoplasmic male sterile (CMS) line of brown cotton comparing with its maintainer line. The result showed that abortion of auxocyte cells in CMS anthers took place during the development periods of sporogneous cells and microspore mother cells (MMC's). Abortion characteristics were abnormal behavior of chromosomes, formation of multi micronucleli in one cell, high frequency of nucleolus penetration through nucleus envelop and cell wall, high vacuolization of cytoplasm and coalescence of MMC's. As compared with the tapetal cells of the fertile anther, those of the sterile anther were significantly smaller and vaculolarer. It was suggested that the abnormality of tapetum development was associated with the abortion of MMC's.

关 键 词：彩色棉 CMS 小孢子 细胞学 细胞质雄性不育花药 

分 类 号：S562.03[农业科学—作物学]