组培环境CO_2增施监控系统的设计与试验  

Design and Experiment of CO_2 Enrichment and Real-time Control System for Tissue Culture

作　　者：丁永前[1] 丁为民[1] 崔瑾[1] 李式军[1] 徐志刚[1] 汪小函[1] 

机构地区：[1]南京农业大学

出　　处：《农业工程学报》2002年第1期96-98,共3页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金项目 (3 983 0 2 3 0 )

摘　　要：为改善组培苗的生长发育环境 ,探索 CO2 富集等环境因素的影响 ,设计制作了 CO2 适时增施监控系统 ,并以葡萄组培苗为对象 ,利用本控制系统与传统组培方式进行了对比试验。结果表明 :系统工作稳定、正常 ,能够有效地将 CO2 浓度控制在设定的 (80 0～ 12 0 0 )μL / L范围内 ,满足组培苗光合作用的需要 ;在 CO2 富集环境中 ,组培苗生长健壮、发育良好 。In order to improve the environmental conditions of tissue culture in vitro and to investigate the function of CO 2 in the culture, a CO 2 enrichment and real time control system was developed. Using this system, the growth experiment of subcultured grape (cabernet sauvignon) plantlets was carried out as compared with the traditional tissue culture method (non CO 2 enrichment). The results indicated that the system worked well, the required ranges of CO 2 concentration [(800～1 200) μL/L] could be obtained; and the CO 2 enrichment environment could promote the growth and photoautotrophic capability of the plantlets in vitro.

关 键 词：CO2富集 实时控制 生物环境 组织培养 无糖培养 增施监控系统 

分 类 号：S625.51[农业科学—园艺学] Q943.1[生物学—植物学]