鸡大肠杆菌多价灭活油乳疫苗的研制与应用  

Preparation and Application of Polyvalent Oil-adjuvant Inactivated Vaccine against Chicken E.coli

作　　者：刁有祥[1] 冯涛[1] 陈庆普[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国预防兽医学报》2002年第1期59-62,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：选择从山东不同地区分离的 6个具有优势血清型的大肠杆菌菌株 ,经培养、混合、灭活后 ,用白油、吐温、司本 ,按一定比例混合 ,制备鸡大肠杆菌多价灭活油乳疫苗 ,安全性检验、效力检验、稳定性试验结果表明 ,该疫苗安全有效 ,接种后无不良反应 ,保护率达 95 %以上 ,4～ 8℃保存 1年 ,2 0～ 2 5℃保存半年不失效。田间免疫试验和扩大区域试验结果表明 ,该疫苗性能良好、安全 ,对蛋鸡、肉鸡、雏鸡的生产性能无明显不良影响 ,经免疫后 ,因大肠杆菌的死亡率由对照组的 7.5 %、9.8%、4.6 %,降至 2 .1%、2 .8%、1.8%。Polyvalent oil_adjuvant inactivated vaccine against chicken E.coli were prepared with six different predominant serotype strains of E.coli which isolated from different regions of Shandong province.The results of safety,immunity and stability tests showed that the vaccine had good safety and induced excellent immune response.It could be store at 4℃～8℃ for more than 12 months and 10℃～25℃ for more than 3 months.The protection rate was 95% and the duration of immunity lasted for 6 months.The results of field test showed that layers,broilers and chickens had no bad affection after injection.The death rate were 2.1%,2.8%,1.8%,while the control groups were 7.5%,9.8%,4.6%respectively.

关 键 词：大肠杆菌 多价灭活疫苗 研制 应用 鸡 

分 类 号：S852.612[农业科学—基础兽医学] S858.315.1[农业科学—兽医学]