红花黄色素对培养人脐静脉血管内皮细胞作用的研究  

Effect of safflor yellew on cultured vascular endothelic cell

作　　者：李文[1] 金鸣[1] 李金荣[1] 路彤[2] 陈铁军[3] 

机构地区：[1]北京市心肺血管疾病研究所细胞免疫室,北京100029 [2]首都医科大学附属安贞医院,北京100029 [3]首都医科大学病理室,北京100054

出　　处：《中国药理学通报》2001年第6期700-701,共2页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金 (No .396 70 884);首都医科大学基础与临床联合科研基金资助课题

摘　　要：目的　观察红花黄色素 (SY)对血管内皮细胞的毒性 ,探讨其对血小板活化因子 (PAF)损伤血管内皮细胞作用的影响。方法　将培养的人脐静脉血管内皮细胞 (HVEC)和EVC 30 4细胞系分别分为PAF、SY、SY +PAF、银杏内酯(ginkolide)和 ginkolide +PAF 5组 ,以光镜和结晶紫比色法观察细胞形态和生物活性变化。结果　HVEC与PAF(10 -11～ 10 -6mol·L-1)孵育 30min后 ,其形态由长梭状或多边形变为椭圆或圆形 ,细胞间隙加大 ,由紧密排列变为疏松的单个细胞 ;HVEC与SY +PAF孵育后 ,形态变化与PAF所致相似 ,生物活性亦见下降趋势 ;HVEC与SY (0 5 71g·L-1)孵育仅 30min即出现形态改变 ,生物活性也有下降趋势 ;EVC 30 4与SY(1 143g·L-1)培养 4h后可致形态改变和生物活性下降。结论　在培养条件下未见SY拮抗PAF损伤内皮细胞的作用 。AIM To study toxicity of Safflor Yellew (SY) for vascular endothelic cell (VEC) and whether SY has antagonistic effect on platelet activating factor (PAF). METHODS Use light microscopical observation and crystal violet colorimetric method to study morphological and bioactivity change of HVEC and EVC 304 after incubated with PAF, SY, SY+PAF, Ginkolide,and Ginkolide+PAF. RESULTS Cultured with PAF 30 minutes, HVEC had histopathological changes: from fusiform, polygond squamous cell to ellipsoid, circular cell , not binding tightly, but separated by wide gap. As cultured with SY+PAF, cell's morphological changes just as same as PAF's group. And its bioactivity had tendency of decline. Either HVEC was incubated with SY (0.571 g·L -1 ) for 30 minutes or EVC 304 cultured with SY(1 143 g·L -1 ) for 4 hours, their morphology and bioactivity had changes. CONCLUSION SY do not inhibit PAF's injury effect. And high concentration SY has toxicity to cultured endothelic cell.

关 键 词：红花黄色素 血管内皮细胞 血小板活化因子 中药 植物药 实验研究 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]