CagA基因阳性Hp培养滤液对人胃粘膜上皮细胞系DNA损伤作用  

Effects of broth culture filtrates of CagA gene positive Helicobacter pylori on DNA damage of human gastric epithelial cell line

作　　者：陈洁平[1] 沈鼎明[2] 杨致邦[3] 高晋华[1] 

机构地区：[1]第三军医大学附属西南医院血液科,重庆400038 [2]重庆医科大学第二临床学院消化内科,重庆400011 [3]重庆医科大学微生物学教研室,重庆400046

出　　处：《第三军医大学学报》2001年第8期928-930,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(39870046);国家博士后基金项目

摘　　要：目的 研究 ＣａｇＡ阳性幽门螺杆菌（Ｈｐ）培养滤液对人胃粘膜上皮细胞（ＧＥＳ－１）ＤＮＡ的损伤作用。方法 制备 Ｈｐ培养滤液，ＰＣＲ鉴定ＣａｇＡ基因。采用单细胞微凝胶电泳观察Ｈｐ（ＣａｇＡ＋）培养滤液对ＧＥＳ－Ｉ细胞ＤＮＡ的损伤作用。结果 Ｈｐ（ＣａｇＡ＋）培养滤液可使ＧＥＳ－Ｉ细胞形成彗星现象。结论 ＨＰ（ＣａｇＡ＋）培养滤液可以导致ＧＥＳ－Ｉ细胞的ＤＮＡ损伤。ＤＮＡ损伤可能是ＣａｇＡ诱导 ＧＥＳ－Ｉ细胞恶性转化的机制之－。Objective To study the effects of broth culture filtrates of CagA gene positive Helicobacter pylori on DNA damage of human gastric epithelial cells line. Methods Broth culture filtrates of Helicobacter pylori was prepared. CagA gene were identified by PCR. GES-I cells were treated with CagA gene positive broth culture filtrates of Helicobacter pylori. Single cell microgel electrophoresis were used to observe the damage of GES-I cell DNA caused by the broth culture filtrates. Results HP(CagA + ) culture filtrates may cause comet phenomenon of GES-I cells. Con- clusion DNA damage can be induced by borth culture filtrates of CagA gene positive Helicobacter pylori in human gastric epithelial cells GES-I line. DNA damage may be one of the mechanism of malignant transformation induced by broth culture filtrates of CagA gene positive Helicobacter py- lori in human gastric epithelial cells.

关 键 词：幽门螺杆菌 CAGA基因 胃粘膜上皮细胞 DNA损伤 HP感染 胃癌 

分 类 号：R378.2[医药卫生—病原生物学] R735.2[医药卫生—基础医学]