随机扩增DNA指纹分型分析耐利福平结核分支杆菌的传播  

Evidence of transmission of M tuberculosis resisting to rifampin by random amplified polymorphic DNA fingerprinting

作　　者：陈炜[1] 钟敏[2] 温博海[1] 陈荣[1] 钟明[2] 娄乐山[2] 王安容[2] 蹇锐[1] 

机构地区：[1]第三军医大学基础医学部微生物学教研室,重庆400038 [2]重庆市肺科医院,重庆400020

出　　处：《第三军医大学学报》2001年第8期942-944,共3页Journal of Third Military Medical University

基　　金：重庆市医学重点资助项目(99-1002);重庆市科委攻关资助项目(2000年)

摘　　要：目的 对临床分离菌株进行鉴定，分析耐利福平结核分支杆菌是否在人群中传播。方法 采用一对随机引物（Ｐ１５’－ＣＣＧＧＧＧＣＣＧＧＴＴＣ；Ｐ２ ５’－ＣＣＧＣＣＣＡＣＣＧＡＣ），对从重庆地区肺结核病人痰标本中分离到的１００株耐利福平结核分支杆菌的 ＤＮＡ进行随机ＰＣＲ扩增，根据扩增产物的ＤＮＡ电泳指纹进行分型。结果１００株耐利福平结核分枝杆菌的ＤＮＡ指纹可分为６种类型，以Ⅱ、Ⅰ和Ⅲ型为主，它们分别占４０％、３１％和２５％；这３型菌株有１／４是从初治结核病人的痰标本中分离，而在２０－３９岁年龄段则有１／３是从初治结核病人的痰标本中分离。结论 随机扩增ＤＮＡ指纹是一种简便、敏感、重复性好的菌株分型方法，可用于耐药性结核病流行监测。重庆地区结核病的流行很可能与耐利福平结核分支杆菌的传播有关。Objective To determine whether Mycobacterium tuberculosis resisting to rifampin transmits among people. Methods The finger- prints of genomic DNA from M tuberculosis strains resisting to rifampin, isolated from sputum specimens of 100 pulmonary tuberculosis patients, were obtained by random amplified polymorphic DNA (RAPD) fingerprinting with primer 1 (5' -CCGGGGCGGTTC) and primer 2 (5'-CCGCCGAC- CGAC). Results Six types of DNA fingerprints were classified; the main types were II, I and III and they were 40%, 31 %, and 25%, respec- tively. Approximately 24% of type I, II, and III strains were isolated from the patients who had received inifial anti-tuberculosis treatment; approxi- mately 31% of the 3 type strains were isolated from the first treated young patients (20-39 year old). Conclusion The RAPD fingerprinting is an useful method to type M tuberculosis strains; the prevalence of tuberculosis resisting to rifampin in Chongqing city may be primarily due to the trans- mission of type II, I and III strains.

关 键 词：结核分支杆菌 利福平 耐药 DNA指纹 结核病 

分 类 号：R378.911[医药卫生—病原生物学] R520.3[医药卫生—基础医学]