高糖腹膜透析液对小鼠腹腔巨噬细胞活力和NO产生的影响  

Effects of high-glucose peritoneal dialysate on viability and nitric oxide production of murine peritoneal macrophages

作　　者：梁国庆[1] 李继承[1] 杨则然[2] 

机构地区：[1]杭州医学高等专科学校形态学实验室,浙江杭州310012 [2]浙江大学医学院细胞生物学和组织工程学研究所

出　　处：《细胞与分子免疫学杂志》2001年第6期582-583,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助No.39970381

摘　　要：目的探讨商业用腹膜透析液commercialperitonealdialysateCDS对腹腔巨噬细胞功能的影响。方法培养的小鼠腹腔巨噬细胞MΦ在含不同葡萄糖浓度15、25和42g/L的CDS中,经不同时间10、30和60min的暴露,观察MΦ还原MTT的能力及其NO的产量。结果低葡萄糖浓度(25g/L)暴露10min实验组,MΦ还原MTT的能力(A570nm值)为0.210±0.008,NO的产量为(9.1±1.3)μmol/L,均明显低于对照组(P<0.01)高葡萄糖浓度(42g/L)和较长时间(60min)暴露组,MΦ还原MTT的能力(A570nm值)为0.056±0.004,NO的产量为(5.7±1.1)μmol/L,与对照组相比较,改变最明显(P<0.01)。结论短时间CDS暴露,即可降低MΦ的活力及NO产生,这种作用与CDS中的葡萄糖浓度以及MΦ暴露于CDS的时间呈正相关。Aim to explore the effect of peritoneal dialysate(CDS)on functions of murine peritoneal macrophages. Methods Cultured peritoneal macrophages were exposed to peritoneal dialysate containing different concentration of glucose (15, 25 and 42 g/L) for different interval times (10 min, 30 min and 60 min). Then the nitric oxide (NO) produced by peritoneal macrophages,and the viability of peritoneal macrophages was tested was observed by MTT method. Results In low concentration of glucose (25 g/L) and 10 min exposing to peritoneal dialysate group, viability(A570nm=0.210±0.008)and nitric oxide production[(9.1±1.3)μmol/L] of macrophages were remarkably lower than those in control group(P<0.01). High concentration of glucose (42.5 g/L ) and 60 min exposing group viability (A570nm=0.056±0.004) and NO production [(5.7±1.1)μmol/L] had obviously changed as compared with the control group(P<0.01). Conclusion Short-time exposing to peritoneal dialysate may decrease viability and NO production of peritoneal macrophages, and these two effects are directly proportional to concentration of glucose in peritoneal dialysate and the interval they exposed to that.

关 键 词：膜腹透析液 腹腔巨噬细胞 一氧化氮 肾功能衰竭 

分 类 号：R692.5[医药卫生—泌尿科学] R983[医药卫生—外科学]