氨溴索对抗低氧性肺动脉高压大鼠慢性肺损伤的保护作用  

Protective effect of ambroxol against the lung damage in chronically hypoxic rats

作　　者：邝土光[1] 张洪玉[1] 庞宝森[1] 牛淑洁[1] 翁心植[1] 张杰[1] 毛燕龄[1] 黄秀霞[1] 

机构地区：[1]首都医科大学附属北京朝阳医院呼吸科北京市呼吸病研究所

出　　处：《中国病理生理杂志》2001年第8期759-761,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨氨溴索对低氧性肺动脉高压大鼠慢性肺损伤的对抗作用。方法 :36只Wistar雄性大鼠 ,随机分成 3组 :正常对照组 ,低氧组 ,氨溴索干预组。后 2组大鼠制成低氧性肺动脉高压模型。检测肺动脉平均压及血液和肺匀浆超氧化物歧化酶 (SOD)活性及过氧化脂质 (LPO)和一氧化氮 (NO)的含量 ,各组随机选取 10只大鼠 ,取其右下肺叶做病理组织切片 ,观察肺组织和肺血管损伤的情况。结果 :低氧组大鼠血液和肺组织SOD和NO水平明显低于正常对照组 ,而血浆和肺组织LPO水平明显高于正常对照组 (P均 <0 0 1) ;氨溴索干预组大鼠血液和肺组织SOD和NO水平明显高于低氧组 ,而血液和肺组织LPO水平明显低于低氧组 (P均 <0 0 1) ,氨溴索干预组肺动脉平均压显著低于低氧组 (P <0 0 1) ;氨溴索干预组的肺动脉平滑肌细胞和细胞外基质的病变明显轻于低氧组 ,管壁面积占总面积的百分比和管壁厚度占外径的百分比明显低于低氧组 (P <0 0 1)。结论 :慢性低氧大鼠体内存在着氧化 /抗氧化失衡 ,可能是肺组织和血管损伤的原因之一 ;氨溴索具有较好的抗氧化能力 。AIM: To investigate the effect of ambroxol on pulmonary and vascular injury in chronically hypoxic rats. METHODS: 36 male Wistar rats were randomly divided into 3 groups: normal control,chronically intermittent hypoxia(CIH) and ambroxol precaution group(AP).The CIH and AP groups were made into the chronically hypoxic models .The mean pulmonary artery pressure(PAPM) and the levels of plasma superoxide dismutase (SOD) and plasma nitric oxide (NO),lipid peroxide(LPO) were determined. The levels of the lung homogenates SOD, LPO, NO and the changes in pulmonary vascular structure were also examined. RESULTS: The levels of plasma and lung homogenates SOD,NO in CIH group were respectively significantly lower than that of normal control and AP group ( P <0.01),but the levels of plasma and lung homogenates LPO were significantly higher( P< 0 01). PAPM in AP group is significantly lower than that of CIH group ( P< 0.01);The damage of pulmonary artery smooth muscle cells and extra cell matrix of AP group is much slighter than that of CIH group. CONCLUSION: Ambroxol might be an effective protector in chronically hypoxic rats.

关 键 词：自由基 抗氧化药 氨溴素 低氧性肺动脉高压 慢性肺损伤 动物实验 

分 类 号：R979.9[医药卫生—药品] R543.2[医药卫生—药学]