癌宁诱导体外人胃腺癌细胞SGC-7901凋亡及细胞周期分析  

Study on Apoptosis of Human Gastric Adenocarcinoma Cell SGC-7901 Induced by Aining in vitro and the Cell Cycle

作　　者：苏勉诚 吕晓英[2] 李红坤[3] 万斌[3] 刘继林[3] 

机构地区：[1]广东省珠海市国家中药现代化工程技术研究中心,珠海519020 [2]四川大学公卫学院,成都610041 [3]成都中医药大学,610075

出　　处：《中药材》2001年第12期876-879,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:探讨中药复方——癌宁诱导人胃腺癌细胞凋亡的作用。方法:采用DNA电泳和流式细胞术,对中药复方——癌宁诱导人胃腺癌细胞凋亡进行了研究。结果:经癌宁处理后的人胃腺癌细胞出现DNA梯状图谱,流式细胞术检测其凋亡细胞百分数高达43.5％,而对照组既无DNA梯状图谱,又无凋亡峰。细胞周期分析表明,经癌宁处理后,细胞的增殖指数为33.7％,无细胞周期特异性。结论:癌宁在体外对人胃腺癌细胞具有诱导凋亡作用。Objective: To study the inducing apoptosis effect of Aining on human gastric adenocarcinoma cell in vitro. Methods: Agarose gel electrophoresis (AGE) and flow cytometry (FMC) techniques were adopted to study the inducing apoptosis effect of Ainging on the human gastric adenocarcinoma cell. Results:The human gastric adenocarcinoma cell's DNA was digested into fragments which gave rise to a characteristic 'DNA Ladder' on agarose gel electrophoresis, while the percentage of apoptotic cells was 43.5% . But the blank group had neither DNA ladder, nor apoptotic peak. Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty. Conclusion:Aining can induce human gastric adenocarcoma cell apoptosis in vitro.

关 键 词：中药复方 癌宁 胃腺癌细胞 细胞凋亡 细胞周期 

分 类 号：R286.91[医药卫生—中药学]