高效液相色谱-荧光检测法测定异丙酚的血药浓度  

Determination of Propofol in Plasma by HPLC with Fluorescence Detection

作　　者：申屠建中[1] 吴丽花[1] 章霞[1] 金旭东[1] 史美甫[1] 

机构地区：[1]浙江大学医学院附属第一医院临床药理研究室,杭州310003

出　　处：《药物分析杂志》2002年第1期65-67,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定异丙酚血药浓度的方法。方法:应用反相高效液相色谱技术,血浆样品经10％三氯乙酸甲醇溶液沉淀后,离心取上清液,以甲醇-水(85:15)为流动相,流速1mL·min^(-1),进样量50μL,经C_(18)柱分离后,荧光检测,λex=276nm,λem=310nm。结果:在0.1～8.0μg·mL^(-1)浓度范围内,异丙酚峰面积与浓度呈良好的线性关系(r=0.9994)。异丙酚的平均加样回收率大于90％,日内和日间RSD均小于4％。本实验最低检测浓度为0.05μg·mL^(-1),绝对回收率大于70％。结论:本法快速、简便、准确、灵敏,可用于异丙酚的血药浓度监测。Objective: To develop a method for determination of propofol in plasma. Method: RP- HPLC was applied to quantitative analysis. Plasma samples were precipitated with 10% trichloroacetic acid methanol solution and then cen-trifugated. 50uuuuuL of supernatant was .injected and separated by Diamonsil C18 column. The mobile phase was composed of methanol - water (85:15), the flow rate was 1 inL·min-1 . The fluorescence detective waves were: λex = 276 nm andλem = 310 nm. Results: There is a good linear relationship within the range of 0.1 -8.0 μg·mL-1 ( r = 0.999 4), and the average recovery was more than 90%. The USD of intra - day and inter - day assays were all less than 4% . The limit of detection proved to be 0.05 μg·mL-1, the absolute recovery was greater than 70% . Conclusion: The method is rapid, simple, accurate and sensitive for blood concentration monitoring of propofol.

关 键 词：异丙酚 高效液相色谱荧光法 血药浓度 药代动力学 

分 类 号：R971.2[医药卫生—药品] R969.1[医药卫生—药学]