模拟失重对流体剪切应力诱导成骨细胞环氧合酶-2表达的影响  

Effects of Simulated Weightlessness on the Expression of Cyclooxygenase-2 in Rat Calvarial Osteoblasts Induced by Fluid Shear Stress

作　　者：张舒[1] 吴兴裕 李莹辉[2] 谢力勤[2] 

机构地区：[1]第四军医大学航空航天生物动力学教研室,陕西西安710032 [2]航天医学工程研究所,北京100094

出　　处：《航天医学与医学工程》2002年第1期27-31,共5页Space Medicine & Medical Engineering

基　　金：第四军医大学创新工程 (CX0 1A0 12 )

摘　　要：目的探讨模拟失重对成骨细胞力学信号转导功能的影响。方法体外传代培养的大鼠乳鼠颅骨成骨细胞分为两组 ,一组置于回转器中培养 ,另一组则在地面重力环境下培养。 60h后 ,将成骨细胞置于流室系统中进行刺激实验。流体剪切应力 (FSS)分别为 0 .5Pa和 1 .5Pa ,作用时间分别为 30min和60min。其后进行免疫组化染色和图像分析。结果在 1G组 ,相同时间不同水平FSS作用诱导的环氧合酶 - 2 (COX 2 )蛋白表达无显著差异 ,FSS作用 30min与 60min所诱导的COX 2蛋白表达差异有显著性意义 (P <0 .0 1 ) ;模拟失重环境下COX 2蛋白表达发生下调性变化 ,仅在 1 .5PaFSS作用 60min的成骨细胞中检测到蛋白表达。相同时间和FSS下 ,模拟失重组与 1G组COX 2蛋白表达水平的差异有显著性意义 (P <0 .0 1 )。Objective To investigate the effect of simulated weightlessness on mechanotransduction in rat calvarial osteoblasts. Method Osteoblasts were isolated from neonatal rat calvariae and then were set to two groups. One was cultured in clinostat to simulate the weightlessness environment. Another was cultured in 1 G gravitational environment. After 60 h, osteoblasts were treated with 0.5 Pa or 1.5 Pa FSS in a flow chamber. The FSS treatment time was set to 30 min and 60 min, respectively. The specimens of each group were obtained for immunohistochemistry staining and determination of expressions of cyclooxygenase 2 (COX 2)in osteoblasts. Result In osteoblasts cultured in 1 G gravitational environment, COX 2 expression was increased remarkably from 30 min to 60 min with the treatment of FSS (P<0.01). There was no clear difference between the expression of COX 2 after treatments of 0.5 Pa or 1.5 Pa FSS; In cells cultured in simulated weightlessness environment, no detectable expression of COX 2 was found with the treatment of 0.5 Pa FSS. The expression of COX 2 was only detected after 60 min treatment of 1.5 Pa FSS, but the level of expression was decreased significantly(P<0.01). Conclusion These results demonstrated that the mechanotransduction in osteoblasts was down regulated in stimulated weightlessness.

关 键 词：失重模拟 流体剪切应力 成骨细胞 前列腺素E2 环氧合酶 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]